Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1, Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Department of Oriental Medicinal Material and Processing College of Life Science, Kyung Hee University, Seocheon-dong, Giheung-gu, Yongin-si, Gyeonggi-do 446-701, Republic of Korea.
Int J Syst Evol Microbiol. 2011 May;61(Pt 5):1123-1126. doi: 10.1099/ijs.0.022087-0. Epub 2010 Jun 11.
A Gram-staining-positive, coccus-shaped, non-spore-forming, facultatively anaerobic bacterium, designated AC-1(T), was isolated from an acidogenic fermentation bioreactor treating food wastewater. On the basis of 16S rRNA gene sequence analysis, strain AC-1(T) was shown to belong to the genus Vagococcus. The closest phylogenetic relatives were Vagococcus elongatus PPC9(T) (97.4 % 16S rRNA gene sequence similarity), Vagococcus penaei CD276(T) (96.7 %) and Vagococcus carniphilus ATCC BAA-640(T) (96.6 %). The major fatty acids were C(18 : 1)ω9c (24.8 %) and C(16 : 0) (19.5 %) and the G+C content of genomic DNA was 44.2 mol%, which supported the affiliation of strain AC-1(T) to the genus Vagococcus. Strain AC-1(T) and V. elongatus DSM 21480(T) exhibited 11 % DNA-DNA relatedness. Physiological and biochemical tests differentiated strain AC-1(T) from the type strains of recognized species of the genus Vagococcus. Therefore, strain AC-1(T) is considered to represent a novel species, for which the name Vagococcus acidifermentans sp. nov. is proposed. The type strain is AC-1(T) ( = KCTC 13418(T) = LMG 24798(T)).
一株革兰氏阳性、球菌状、非孢子形成、兼性厌氧细菌，命名为 AC-1(T)，从处理食品废水的产酸发酵生物反应器中分离得到。基于 16S rRNA 基因序列分析，菌株 AC-1(T)属于 V agococcus 属。最接近的系统发育相关菌为 Vagococcus elongatus PPC9(T)(97.4% 16S rRNA 基因序列相似性)、Vagococcus penaei CD276(T)(96.7%)和 Vagococcus carniphilus ATCC BAA-640(T)(96.6%)。主要脂肪酸为 C(18:1)ω9c(24.8%)和 C(16:0)(19.5%)，基因组 DNA 的 G+C 含量为 44.2mol%，这支持菌株 AC-1(T)属于 V agococcus 属。菌株 AC-1(T)和 V. elongatus DSM 21480(T)的 DNA-DNA 同源性为 11%。生理生化试验将菌株 AC-1(T)与公认的 V agococcus 属种的模式株区分开来。因此，菌株 AC-1(T)被认为代表一个新的种，建议命名为 Vagococcus acidifermentans sp. nov.。其模式菌株为 AC-1(T)(=KCTC 13418(T)=LMG 24798(T))。
