Marrazzo A, La Bara G, Taormina P, Bazan P
Clinica Chirurgica III e Centro Interdipartimentale di Ricerca in Oncologica Clinica, Università di Palermo, Italy.
Br J Cancer. 1989 Mar;59(3):426-8. doi: 10.1038/bjc.1989.86.
Fifty patients with operable breast carcinoma underwent fine needle aspiration for cytological examination. The smears were prepared by means of the immunocytochemical method using monoclonal antibodies for the determination of the oestrogen receptors (ER). After surgery the contents of the ER were determined with the traditional biochemical technique. The results of the immunocytochemical method showed 31 positives, two of which disagreed with the biochemical results, 15 negatives and four cases which could not be assessed due to the absence of adequate numbers of cells. The ICA staining for ER was expressed on a semiquantitative basis; there was a significant correlation between this and the values expressed by the biochemical technique, with a coefficient of 0.83, P less than 0.000006.
五十例可手术乳腺癌患者接受了细针穿刺进行细胞学检查。涂片采用免疫细胞化学方法，使用单克隆抗体来测定雌激素受体（ER）。手术后，用传统生化技术测定ER含量。免疫细胞化学方法的结果显示31例阳性，其中2例与生化结果不一致，15例阴性，4例因细胞数量不足无法评估。ER的免疫细胞化学染色采用半定量方式表达；其与生化技术所表达的值之间存在显著相关性，系数为0.83，P小于0.000006。
