Omotoso Gabriel Olaiya, Gbadamosi Ismail Temitayo, Olajide Olayemi Joseph, Dada-Habeeb Shakirat Opeyemi, Arogundade Tolulope Timothy, Yawson Emmanuel Olusola
Division of Neurobiology, Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, University of Ilorin, P.M.B. 1515, Ilorin, Nigeria.
Division of Neurobiology, Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, University of Ilorin, P.M.B. 1515, Ilorin, Nigeria.
Pathophysiology. 2018 Mar;25(1):57-62. doi: 10.1016/j.pathophys.2017.12.003. Epub 2018 Jan 4.
Nicotine is a neuro-stimulant that has been implicated in the pathophysiology of many brain diseases. The need to prevent or alleviate the resulting dysfunction is therefore paramount, which has also given way to the use of medicinal plants in the management of brain conditions. This study was designed to determine the histomorphological and neurobehavioural changes in the cerebellum of Wistar rats following nicotine insult and how such injuries respond to Moringa intervention. Twenty-four adult male Wistar rats were divided into 4 groups. Group A and B were orally treated with normal saline and Moringa oleifera respectively for twenty-eight days; Group C was treated with nicotine while group D was treated orally with Moringa oleifera and intraperitoneally with nicotine for twenty-eight days. Animals were subjected to the open field test on the last day of treatment. 24 h after last day treatment, the animals were anesthetized and perfusion fixation was carried out. The cerebellum was excised and post-fixed in 4% paraformaldehyde and thereafter put through routine histological procedures. Results revealed cytoarchitectural distortion and extreme chromatolysis in neuronal cells of the cerebellar cortical layers in the nicotine-treated group. The Purkinje cells of the cerebellum of animals in this group were degenerated. There were also reduced locomotor activities in the group. Moringa was able to prevent the chromatolysis, distortion of the cerebellar cortical cells and neurobehavioural deficit. Our result suggests that Moringa oleifera could prevent nicotine-induced cerebellar injury in Wistar rats, with the possibility of ameliorating the clinical features presented in associated cerebellar pathology.
尼古丁是一种神经兴奋剂，与多种脑部疾病的病理生理学有关。因此，预防或减轻由此产生的功能障碍至关重要，这也使得药用植物在脑部疾病的治疗中得到应用。本研究旨在确定尼古丁损伤后Wistar大鼠小脑的组织形态学和神经行为变化，以及这种损伤如何对辣木干预作出反应。将24只成年雄性Wistar大鼠分为4组。A组和B组分别口服生理盐水和辣木，持续28天；C组用尼古丁治疗，而D组口服辣木并腹腔注射尼古丁，持续28天。在治疗的最后一天对动物进行旷场试验。在最后一天治疗后24小时，将动物麻醉并进行灌注固定。切除小脑并在4%多聚甲醛中后固定，然后进行常规组织学程序。结果显示，尼古丁治疗组小脑皮质层神经元细胞出现细胞结构扭曲和严重的染色质溶解。该组动物小脑的浦肯野细胞发生退化。该组动物的运动活动也减少。辣木能够防止染色质溶解、小脑皮质细胞扭曲和神经行为缺陷。我们的结果表明，辣木可以预防Wistar大鼠尼古丁诱导的小脑损伤，有可能改善相关小脑病理学中出现的临床特征。
