Lin Mengfei, Ma Shiying, Quan Kehui, Yang Endian, Hu Lei, Chen Xiaoyang
Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha, China 410001.
State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, China 510642.
Physiol Mol Biol Plants. 2022 May;28(5):935-946. doi: 10.1007/s12298-022-01186-4. Epub 2022 May 21.
, is commonly cultivated as a vegetable in tropical and subtropical regions because of nutritional and medicinal benefits of its fruits, immature pods, leaves, and flowers. Flowering at the right time is one of the important traits for crop yield in . Under normal conditions, photoperiod is one of the key factors in determining when plant flower. However, the molecular mechanism underlying the effects of a long-day photoperiod on is not clearly understood. In the present study, deep RNA sequencing and sugar metabolome were conducted of leaves under long-day photoperiod. As a result, differentially expressed genes were significantly associated with starch and sucrose pathway and the circadian rhythm-plant pathway. In starch and sucrose pathway, sucrose, fructose, trehalose, glucose, and maltose exhibited pronounced rhythmicity over 24 h, and (trehalose-6-phosphate synthase) genes constituted key regulatory genes. In an overexpression line hosting the or genes, flowering occurred earlier under a short-day photoperiod. These results will support molecular breeding of and may help clarify to genetic architecture of long-day photoperiod related traits.
The online version contains supplementary material available at 10.1007/s12298-022-01186-4.
由于其果实、未成熟豆荚、叶子和花朵具有营养和药用价值，[植物名称]在热带和亚热带地区常作为蔬菜种植。适时开花是[植物名称]作物产量的重要性状之一。在正常条件下，光周期是决定植物何时开花的关键因素之一。然而，长日照光周期对[植物名称]影响的分子机制尚不清楚。在本研究中，对长日照光周期下的[植物名称]叶片进行了深度RNA测序和糖代谢组分析。结果表明，差异表达基因与淀粉和蔗糖途径以及昼夜节律-植物途径显著相关。在淀粉和蔗糖途径中，蔗糖、果糖、海藻糖、葡萄糖和麦芽糖在24小时内呈现出明显的节律性，[海藻糖-6-磷酸合酶]基因构成关键调控基因。在携带[基因名称]或[基因名称]基因的[植物名称]过表达系中，短日照光周期下开花提前。这些结果将为[植物名称]的分子育种提供支持，并可能有助于阐明长日照光周期相关性状的遗传结构。
在线版本包含可在10.1007/s12298-022-01186-4获取的补充材料。
