College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, Zhejiang Province, China.
College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, Zhejiang Province, China; Key Lab of Wildlife Biotechnology, Conservation and Utilization of Zhejiang Province, Zhejiang Normal University, Jinhua, Zhejiang Province, China.
Int J Biol Macromol. 2018 May;111:787-795. doi: 10.1016/j.ijbiomac.2018.01.016. Epub 2018 Jan 5.
We acquired 21 complete mitogenomes and 6 nearly complete mitogenomes of mantises belonging to 8 families (Hymenopodidae, Iridopterygidae, Mantidae, Metallyticidae, Sibyllidae, Tarachodidae, Thespidae, Toxoderidae) using 14 pairs of mantid specific primer sets and found that 5 species of mantises have duplicate copies (2-4) of trnR: Ambivia undata, Creobroter jiangxiensis, Creobroter urbanus, Phyllothelys sp1. and Theopropus elegans while two novel gene arrangements CR-I-NCR-I-NCR-I-NCR-I-NCR-I-NCR*-Q-M and COII-K*-D*-K-D*-K-D*-K-D were found in Schizocephala bicornis and Stenotoxodera porioni, respectively. The multiple copies of trnR are caused by independent duplications. The gene arrangements in Stenotoxodera porioni with three identical copies of trnK can be explained as mid-way through the TDRL process while the form of gene arrangement in Schizocephala bicornis is unclear. In the phylogeny at the family level, the monophyly of Liturgusidae and Iridopterygidae was supported, whereas the monophyly of Hymenopodidae, Mantidae and Tarachodidae wasn't. The features of mantis mitochondrial genomes including high duplication rates of trnR, trnK and trnI indicate that Mantodea mitochondrial genomes maybe a useful model system for studying gene duplication. However, derived gene arrangements may not be appropriate for phylogenetic inference in Mantodea as they aren't synapomorphy and aren't shared by close relatives.
我们使用 14 对螳螂特异性引物对，获得了 21 条完整的线粒体基因组和 6 条近乎完整的线粒体基因组，这些基因组来自 8 个科（螳科、叶脩科、螳科、花螳科、菱蝗科、驼蝗科、花螳科、瘦翅蝗科），结果发现 5 种螳螂具有 trnR 的重复副本（2-4 个）：Ambivia undata、Creobroter jiangxiensis、Creobroter urbanus、Phyllothelys sp1. 和 Theopropus elegans，而在 Schizocephala bicornis 和 Stenotoxodera porioni 中分别发现了两个新的基因排列 CR-I-NCR-I-NCR-I-NCR-I-NCR-I-NCR*-Q-M 和 COII-K*-D*-K-D*-K-D*-K-D。trnR 的多个副本是由独立的重复产生的。 Stenotoxodera porioni 中 trnK 的三个相同副本的基因排列可以解释为 TDRL 过程中途，而 Schizocephala bicornis 的基因排列形式尚不清楚。在科水平的系统发育中，Liturgusidae 和 Iridopterygidae 的单系性得到支持，而 Hymenopodidae、Mantidae 和 Tarachodidae 的单系性则没有得到支持。螳螂线粒体基因组的特征，包括 trnR、trnK 和 trnI 的高重复率，表明 Mantodea 线粒体基因组可能是研究基因重复的有用模型系统。然而，衍生的基因排列可能不适合在螳螂目中进行系统发育推断，因为它们不是同源特征，也不是近亲所共有的。
