Gheber L, Priel Z
Chemistry Department, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
Biophys J. 1989 Jan;55(1):183-91. doi: 10.1016/S0006-3495(89)82790-0.
A novel quantitative parameter is proposed to define and measure the degree of synchronization between two small ciliary areas. These areas can be close to or far from one another. The Pearson correlation factor is used to define the degree of synchronization by a single number. This approach is based on a computerized, dual photoelectric method which simulataneously measures the scattered light from two small areas on the ciliary epithelium or its tissue culture. The measurements were performed on tissue culture from frog's palate epithelium. It was found that: (a) the degree of synchronization decreases, as a function of distance; (b) the correlation is fairly high even at relatively large separations, when measured on the same patch; (c) on a given patch the synchronization factor is independent of the direction of the metachronal wave; (d) close disconnected ciliary cells exhibit fairly high correlation; (e) disconnected randomly choosen ciliary cells at relatively large separation distances exhibit relatively low correlation, smaller by a factor of 2 than the correlation factor at the same distances when measured along the metachronal wave; (f) the average frequencies' ratio and the metachronal wavelength can be used as first-order indicators of ciliary synchronization; (g) there is a spread of metachronal wavelengths even over a single well-organized patch.
提出了一种新的定量参数来定义和测量两个小纤毛区域之间的同步程度。这些区域可以彼此靠近或远离。皮尔逊相关系数用于通过一个单一数字来定义同步程度。该方法基于一种计算机化的双光电方法，该方法同时测量来自睫状上皮或其组织培养物上两个小区域的散射光。测量是在青蛙腭上皮的组织培养物上进行的。结果发现：(a)同步程度随距离的增加而降低；(b)在同一小块区域上测量时，即使在相对较大的间距下，相关性也相当高；(c)在给定的小块区域上，同步因子与顺时波的方向无关；(d)紧密相连但不连续的睫状细胞表现出相当高的相关性；(e)在相对较大的分离距离处随机选择的不连续睫状细胞表现出相对较低的相关性，比沿顺时波测量时相同距离处的相关系数小2倍；(f)平均频率比和顺时波长可作为睫状同步的一阶指标；(g)即使在一个组织良好的小块区域上，顺时波长也存在差异。
