从英国污水样本中分离出类似疫苗的脊髓灰质炎病毒株。

Isolation of Vaccine-Like Poliovirus Strains in Sewage Samples From the United Kingdom.

机构信息

Division of Virology, National Institute for Biological Standards and Control, South Mimms.

Enterovirus Unit, Public Health England, London, United Kingdom.

出版信息

J Infect Dis. 2018 Mar 28;217(8):1222-1230. doi: 10.1093/infdis/jix667.

DOI:10.1093/infdis/jix667

PMID:29309594

Abstract

BACKGROUND

Environmental surveillance (ES) is a sensitive method for detecting human enterovirus (HEV) circulation, and it is used worldwide to support global polio eradication. We describe a novel ES approach using next-generation sequencing (NGS) to identify HEVs in sewage samples collected in London, United Kingdom, from June 2016 to May 2017.

METHODS

Two different methods were used to process raw sewage specimens: a 2-phase aqueous separation system and size exclusion by filtration and centrifugation. HEVs were isolated using cell cultures and analyzed using NGS.

RESULTS

Type 1 and 3 vaccine-like poliovirus (PV) strains were detected in samples collected from September 2016 through January 2017. NGS analysis allowed us to rapidly obtain whole-genome sequences of PV and non-PV HEV strains. As many as 6 virus strains from different HEV serotypes were identified in a single cell culture flask. PV isolates contained only a small number of mutations from vaccine strains commonly seen in early isolates from vaccinees.

CONCLUSIONS

Our ES setup has high sensitivity for polio and non-PV HEV detection, generating nearly whole-genome sequence information. Such ES systems provide critical information to assist the polio eradication endgame and contribute to the improvement of our understanding of HEV circulation patterns in humans.

摘要

背景

环境监测（ES）是一种检测人类肠道病毒（HEV）传播的敏感方法，它被广泛用于支持全球根除脊髓灰质炎行动。我们描述了一种使用下一代测序（NGS）在英国伦敦收集的污水样本中识别 HEV 的新型 ES 方法，采集时间为 2016 年 6 月至 2017 年 5 月。

方法

使用两种不同的方法处理原始污水标本：两相水分离系统和过滤与离心的大小排除。使用细胞培养物分离 HEV 并进行 NGS 分析。

结果

2016 年 9 月至 2017 年 1 月采集的样本中检测到 1 型和 3 型疫苗样脊髓灰质炎病毒（PV）株。NGS 分析使我们能够快速获得 PV 和非 PV HEV 株的全基因组序列。在单个细胞培养瓶中鉴定出多达 6 种不同 HEV 血清型的病毒株。PV 分离株仅包含少数常见于疫苗接种者早期分离株中的疫苗株突变。