Huang J K, Bartsch W, Voigt K D
Acta Endocrinol (Copenh). 1985 Aug;109(4):569-76. doi: 10.1530/acta.0.1090569.
Male Wistar rats were castrated and implantated with testosterone-filled silastic depots (in vitro release rate: 60 micrograms/24 h) prior to treatment with 10 mg cyproterone acetate (CyAc) on day 1, and 5 mg on days 4, 7, 10 and 13. Animals were sacrificed on day 14. Control animals were treated identically, with the exception of CyAc administration. Blood was collected and the ventral prostates of 6-8 animals were pooled, homogenized and processed into cytosol and purified nuclei. Steroid determinations (CyAc, testosterone, 5 alpha-dihydrotestosterone (DHT), 5 alpha-androstane-3 alpha,17 beta-diol (3 alpha-diol)) were performed by RIA. Specifically bound DHT (charcoal resistant DHT = CR-DHT) represents DHT values (RIA) following treatment of cytosol or nuclear extract with dextran coated charcoal. The androgen receptor was determined in cytosol and nuclear extract by 'exchange assay' using [3H]methyltrienolone (MT) (18 h, 15 degrees C). The main results were: 1) The steroid levels in plasma (testosterone, DHT, 3 alpha-diol) were in the range of untreated adult animals and not significantly influenced by the CyAc treatment. Final CyAc levels were 305 +/- 58 nmol/l (mean +/- SD, n = 5).(ABSTRACT TRUNCATED AT 250 WORDS)
雄性Wistar大鼠被阉割，并在第1天用10毫克醋酸环丙孕酮（CyAc）治疗前植入充满睾酮的硅橡胶储库（体外释放率：60微克/24小时），第4、7、10和13天用5毫克。动物在第14天处死。对照动物除不给予CyAc外，处理方式相同。采集血液，将6 - 8只动物的腹侧前列腺合并，匀浆并处理成胞质溶胶和纯化的细胞核。通过放射免疫分析（RIA）进行类固醇测定（CyAc、睾酮、5α - 双氢睾酮（DHT）、5α - 雄甾烷 - 3α,17β - 二醇（3α - 二醇））。特异性结合的DHT（抗活性炭DHT = CR - DHT）代表用葡聚糖包被活性炭处理胞质溶胶或核提取物后的DHT值（RIA）。通过使用[3H]甲基三烯olone（MT）（18小时，15摄氏度）的“交换测定法”在胞质溶胶和核提取物中测定雄激素受体。主要结果如下：1）血浆中的类固醇水平（睾酮、DHT、3α - 二醇）处于未治疗成年动物的范围内，且未受CyAc治疗的显著影响。最终的CyAc水平为305±58纳摩尔/升（平均值±标准差，n = 5）。（摘要截断于250字）
