Lefebvre Y A, Golsteyn E J, Michiel T L
J Steroid Biochem. 1985 Jul;23(1):107-13. doi: 10.1016/0022-4731(85)90268-7.
Intact nuclear envelopes containing nuclear pore complexes have been prepared from the rat ventral prostate. The polypeptide profile of the nuclear envelopes from the rat prostate resembled that of nuclear envelopes prepared from the male rat liver. Isolation of the nuclear envelopes after incubation of purified nuclei with radioactive dihydrotestosterone results in labelling of the membrane. More dihydrotestosterone is bound after incubations at 22 degrees C for 18 h than at 2 degrees C for 18 h or 22 degrees C for 2 h. Scatchard analysis revealed a class of binding sites with an apparent Kd of 46 nM. Dihydrotestosterone, testosterone, cyproterone acetate and methyltrienolone were effective as competitors of labelled dihydrotestosterone binding to the nuclear envelopes, while estradiol did not compete. Castration of the rats 24, 48 and 96 h prior to preparation of nuclei resulted in loss of androgen binding to the membranes. Extraction with 0.6 M NaCl resulted in the loss of 72% of the androgen binding.
已从大鼠腹侧前列腺制备出含有核孔复合体的完整核膜。大鼠前列腺核膜的多肽谱与雄性大鼠肝脏制备的核膜相似。用放射性双氢睾酮孵育纯化的细胞核后分离核膜，会使膜带上标记。在22℃孵育18小时后结合的双氢睾酮比在2℃孵育18小时或22℃孵育2小时后更多。Scatchard分析显示一类结合位点，其表观解离常数为46 nM。双氢睾酮、睾酮、醋酸环丙孕酮和甲基三烯醇酮可有效竞争标记的双氢睾酮与核膜的结合，而雌二醇无竞争作用。在制备细胞核前24、48和96小时对大鼠进行去势，导致雄激素与膜的结合丧失。用0.6 M NaCl提取导致72%的雄激素结合丧失。
