基于纳米生物素化脂质体的免疫分析用于尿液中蛋白质生物标志物的超灵敏检测。
Nano-biotinylated liposome-based immunoassay for the ultrasensitive detection of protein biomarker in urine.
机构信息
Department of Laboratory Diagnosis, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai 200433, China.
Department of Chemistry and Institute of Biomedical Sciences, Fudan University, 220 Handan Road, Shanghai 200433, China.
出版信息
Talanta. 2018 Mar 1;179:472-477. doi: 10.1016/j.talanta.2017.11.031. Epub 2017 Nov 21.
With the development of proteomics and the continuous discovery of biomarkers of trace proteins, it is important to accurately quantify low abundance protein, especially in urine for clinical diagnostics. In this paper, we reported a novel nano-biotinylated liposome-based immuno-loop-mediated isothermal amplification (LI-LAMP) for the ultrasensitive detection of REG1A (a biomarker for pancreatic ductal adenocarcinoma (PDAC) in urine) with high specificity. The detection range was 1µg/mL to 1fg/mL, with a detection limit of 1fg/mL, and no cross-reactivity was observed to occur in this assay. Compared with the amount of REG1A added, REG1A recovery using this method was 130% and 89%. Detection of REG1A concentrations using the LI-LAMP assay from real samples were in good agreement with those determined using ELISA, and relative deviations were not more than 10%. LI-LAMP shows good potential as a clinical diagnostic assay.
随着蛋白质组学的发展和痕量蛋白生物标志物的不断发现，准确地定量低丰度蛋白质，特别是在尿液中进行临床诊断，变得非常重要。本文报道了一种基于纳米生物素化脂质体的免疫环介导等温扩增（LI-LAMP）方法，用于超灵敏检测尿液中的 REG1A（胰腺导管腺癌（PDAC）的生物标志物），具有高特异性。检测范围为 1μg/mL 至 1fg/mL，检测限为 1fg/mL，在该测定中未观察到交叉反应。与添加的 REG1A 量相比，该方法对 REG1A 的回收率为 130%和 89%。使用 LI-LAMP 测定法从实际样本中检测 REG1A 浓度与使用 ELISA 测定的结果非常吻合，相对偏差不超过 10%。LI-LAMP 显示出作为临床诊断检测方法的良好潜力。
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