College of Chemistry, Sichuan University, Chengdu 610064, China.
College of Chemistry, Sichuan University, Chengdu 610064, China.
Talanta. 2018 Mar 1;179:641-645. doi: 10.1016/j.talanta.2017.11.065. Epub 2017 Nov 29.
It is known that DNA molecules can be used to build a various of complicated geometrical DNA nanostructures with programmable sequence design, and these DNA nanomaterials show a promising application in biotechnology and biomedicine. However, the construction of large-sized three dimensional DNA-based nanomaterials still remains a challenge. In this work, we propose a new strategy that only employs one target DNA to trigger multiple catalyzed hairpin assembly (CHA) reactions and sticky ends self-assembly to prepare hundreds of nanometer-sized DNA nanoparticles. Moreover, the obtained DNA nanoparticles can be served as efficient biosensors for sensitive colorimetric nucleic acids detection with a detection limit of 7.7pM.
已知 DNA 分子可以通过可编程序列设计来构建各种复杂的几何 DNA 纳米结构，这些 DNA 纳米材料在生物技术和生物医学中有广阔的应用前景。然而，构建大型三维基于 DNA 的纳米材料仍然是一个挑战。在这项工作中，我们提出了一种新策略，仅使用一个目标 DNA 来触发多个催化发夹组装 (CHA) 反应和粘性末端自组装，以制备数百纳米大小的 DNA 纳米颗粒。此外，所获得的 DNA 纳米颗粒可用作高效的生物传感器，用于灵敏的比色核酸检测，检测限低至 7.7pM。
