Sipes John M, Murphy-Ullrich Joanne E, Roberts David D
Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, United States.
University of Alabama at Birmingham, Birmingham, AL, United States.
Methods Cell Biol. 2018;143:347-369. doi: 10.1016/bs.mcb.2017.08.021. Epub 2017 Nov 6.
Thrombospondins are a family of five secreted proteins that have diverse roles in modulating cellular function. Thrombospondins-1 and 2 were identified as matricellular proteins based on their functional roles combined with their transient appearance or accumulation in extracellular matrix at specific times during development and in response to injury or stress in mature tissues. Thrombospondin-1 is a major component of platelet α-granules, which provides a convenient source for purification of the protein. Methods are described to prepare thrombospondin-1 from human platelets in a biologically active form with minimal degradation or contamination with other platelet proteins. A nondenaturing method is described for removing bound transforming growth factor-β1.
血小板反应蛋白是一个由五种分泌蛋白组成的家族，在调节细胞功能方面具有多种作用。基于其功能作用以及在发育过程中的特定时间或成熟组织对损伤或应激的反应时，它们在细胞外基质中的短暂出现或积累，血小板反应蛋白-1和-2被鉴定为基质细胞蛋白。血小板反应蛋白-1是血小板α-颗粒的主要成分，这为该蛋白的纯化提供了便利来源。本文描述了从人血小板中制备具有生物活性形式的血小板反应蛋白-1的方法，该方法能使蛋白降解或受其他血小板蛋白污染降至最低。还描述了一种用于去除结合的转化生长因子-β1的非变性方法。
