Department of the Sixth Medicine, Guangzhou General Hospital of Guangzhou Military Command, Guangdong Province, China.
Cancer Research Institute, Southern Medical University, Guangdong Province, China.
Sci Rep. 2018 Jan 8;8(1):37. doi: 10.1038/s41598-017-18280-3.
Long non-coding RNAs (lncRNAs) are important regulatory factors in tumor development and progression. The lncRNA CASC9.5 is located on chromosome 8 and has a total length of 1316 bp. CASC9.5 plays a tumor-promoting role in the development and progression of brain tumor and colon cancer; however, limited research has been conducted on the role of this lncRNA in lung adenocarcinoma. The present study analyzed 44 lung adenocarcinoma specimens and 2 lung cancer cell lines. It was found that CASC9.5 expression levels were significantly higher in lung cancer tissues and cells compared with normal lung tissues. In addition, the expression level of CASC9.5 was closely related to the TNM (tumor, node and metastasis) stage of lung adenocarcinomas, tumor size, tumor metastasis and tumor metabolism. Moreover, results of the in vivo and in vitro experiments all demonstrated that CASC9.5 promoted lung adenocarcinoma cell proliferation and metabolism by regulating the expression levels of cyclin D1, E-cadherin, N-cadherin and β-catenin. In summary, the present study demonstrated that high levels of CASC9.5 expression promote the proliferation, metastasis and metabolism of lung adenocarcinoma cells and might serve as a prognostic indicator. The present study provides novel findings regarding the diagnosis and treatment of lung adenocarcinoma.
长链非编码 RNA（lncRNA）是肿瘤发生和发展的重要调节因子。lncRNA CASC9.5 位于 8 号染色体上，全长 1316bp。CASC9.5 在脑肿瘤和结肠癌的发生和发展中发挥着促进肿瘤的作用；然而，关于这种 lncRNA 在肺腺癌中的作用的研究还很有限。本研究分析了 44 例肺腺癌标本和 2 种肺癌细胞系。结果发现，CASC9.5 在肺癌组织和细胞中的表达水平明显高于正常肺组织。此外，CASC9.5 的表达水平与肺腺癌的 TNM（肿瘤、淋巴结和转移）分期、肿瘤大小、肿瘤转移和肿瘤代谢密切相关。此外，体内和体外实验的结果均表明，CASC9.5 通过调节细胞周期蛋白 D1、E-钙黏蛋白、N-钙黏蛋白和β-连环蛋白的表达水平，促进肺腺癌细胞的增殖和代谢。综上所述，本研究表明高水平的 CASC9.5 表达促进了肺腺癌细胞的增殖、转移和代谢，可能成为一种预后指标。本研究为肺腺癌的诊断和治疗提供了新的发现。
