Ren Yongzhe, Qian Yingying, Xu Yanhua, Zou ChunQin, Liu Dongcheng, Zhao Xueqiang, Zhang Aimin, Tong Yiping
State Key Laboratory of Wheat and Maize Crop Science, College of Agronomy, Collaborative Innovation Center of Henan Grain Crops, Henan Agricultural University, Zhengzhou, China.
China Agricultural University, Beijing, China.
Front Plant Sci. 2017 Dec 11;8:2096. doi: 10.3389/fpls.2017.02096. eCollection 2017.
Root is important in acquiring nutrients from soils. Developing marker-assisted selection for wheat root traits can help wheat breeders to select roots desirable for efficient acquisition of nutrients. A recombinant inbred line (RIL) population derived from wheat varieties Xiaoyan 54 and Jing 411 was used to detect QTLs for maximum root length and root dry weight (RDW) under control, low nitrogen and low phosphorus conditions in hydrophobic culture (HC). We totally detected 17 QTLs for the investigated root traits located at 13 loci on 11 chromosomes. These loci differentially expressed under different nutrient supplying levels. The RILs simultaneously harboring positive alleles or negative alleles of the most significant three QTLs for RDW, , and , were selected for soil column culture (SC) trial to verify the effects of these QTLs under soil conditions. The RILs pyramiding the positive alleles not only had significantly higher shoot dry weight, RDW, nitrogen and phosphorus uptake in all the three treatments of the HC trial, but also had significantly higher RDW distribution in both the top- and sub-soils in the SC trial than those pyramiding the negative alleles. These results suggested that QTL analysis based on hydroponic culture can provide useful information for molecular design of wheat with large and deep root system.
根系对于从土壤中获取养分至关重要。开发针对小麦根系性状的标记辅助选择方法，有助于小麦育种者选择有利于高效获取养分的根系。以小麦品种小偃54和京411构建的重组自交系（RIL）群体，用于在疏水培养（HC）条件下，检测对照、低氮和低磷条件下最大根长和根干重（RDW）的QTL。我们共检测到17个与所研究根系性状相关的QTL，分布在11条染色体的13个位点上。这些位点在不同养分供应水平下差异表达。选择同时携带RDW最显著的三个QTL的正向或负向等位基因的RIL进行土柱培养（SC）试验，以验证这些QTL在土壤条件下的效应。在HC试验的所有三种处理中，聚合正向等位基因的RIL不仅地上部干重、RDW、氮和磷吸收显著更高，而且在SC试验中，其在表土和底土中的RDW分布也显著高于聚合负向等位基因的RIL。这些结果表明，基于水培的QTL分析可为根系庞大且深扎的小麦分子设计提供有用信息。
