Assessment of Real-Time Polymerase Chain Reaction for the Detection of spp. DNA from Human Fecal Samples.

Sporadic cases of Tricostrongylosis are reported in humans. Diagnosis of enteric relies primarily on coproscopic analysis but morphological identification is difficult because of similarity among nematode species. The method is time consuming and requires some expertise. To overcome these limitations, we developed a molecular approach by real-time polymerase chain reaction (PCR) to provide a rapid, specific, and sensitive tool to detect spp. in human feces. We designed primers and probe specific for rDNA region 5.8S and internal transcribed spacer 2. Three Italian family clusters were analyzed and DNA sequencing was performed to confirm real-time PCR results comparing with known GenBank sequence data. Sequence analysis showed ≥ 99% identity to and . This study provides a molecular methodology suitable for fast and specific detection of in fecal specimens and to distinguish the zoonotic species.