Milhes M, Guillerm M, Robin M, Eichstadt M, Roy C, Grisez C, Prévot F, Liénard E, Bouhsira E, Franc M, Jacquiet P
UMR INRA/DGER 1225 Interactions Hôtes Agents Pathogènes, UMT Santé des Petits Ruminants, Ecole Nationale Vétérinaire de Toulouse, BP 87614, 31076, Toulouse Cedex 03, France.
Groupement Corrézien de Défense Sanitaire (GCDS), 19001, Tulle Cedex, France.
Parasitol Res. 2017 Mar;116(3):909-920. doi: 10.1007/s00436-016-5364-z. Epub 2017 Jan 18.
Resistance to fenbendazole, ivermectin, and moxidectin was explored by a fecal egg count reduction test in four meat sheep flocks in southwestern France where anthelmintic resistance was suspected. The FECR test results of the present study confirmed the presence of benzimidazole resistance in three out of the four farms and the presence of ivermectin resistance in one flock. In addition, a suspicion of moxidectin resistance was shown in this latter farm. Both conventional morphological and molecular identifications were performed on larval cultures before and after the treatment in the studied farms. A high positive correlation was found between the number of larvae counted under binocular microscope and the number of larvae estimated by the qPCR analysis (R = 0.88) and a high Cohen's Kappa value (0.91) in the detection of strongylid larvae in larval cultures. According to qPCR results, Trichostrongylus species demonstrated high levels of BZ resistance and Teladorsagia circumcincta was involved in the IVM resistance in one farm. The molecular procedures used in this study have the potential to be beneficial for anthelmintic resistance surveillance in sheep industry.
在法国西南部四个怀疑存在抗蠕虫药耐药性的肉羊养殖场，通过粪便虫卵计数减少试验探究了对芬苯达唑、伊维菌素和莫西菌素的耐药性。本研究的粪便虫卵计数减少试验结果证实，四个养殖场中有三个存在苯并咪唑耐药性，一个养殖场存在伊维菌素耐药性。此外，在后一个养殖场显示出对莫西菌素耐药性的怀疑。在研究的养殖场处理前后，对幼虫培养物进行了传统形态学和分子鉴定。在双目显微镜下计数的幼虫数量与通过qPCR分析估计的幼虫数量之间发现高度正相关（R = 0.88），并且在幼虫培养物中检测圆线虫幼虫时科恩kappa值较高（0.91）。根据qPCR结果，毛圆线虫属物种表现出高水平的苯并咪唑耐药性，在一个养殖场中，环形泰勒虫与伊维菌素耐药性有关。本研究中使用的分子程序可能有利于绵羊产业的抗蠕虫药耐药性监测。
