Department of Medical Parasitology and Mycology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
Department of Biostatistics, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
Front Cell Infect Microbiol. 2021 Oct 13;11:759396. doi: 10.3389/fcimb.2021.759396. eCollection 2021.
Human trichostrongyliasis is a zoonotic disease that is prevalent among rural populations in some countries. This study was performed to evaluate various parasitological methods and polymerase chain reaction (PCR) for the diagnosis of human trichostrongyliasis. A total of 206 fresh stool samples were collected from residents of endemic villages of Northern Iran. All samples were examined using conventional parasitological methods, including wet mount, formalin ethyl acetate concentration (FEAC), agar plate culture (APC), Harada-Mori culture (HMC), and Willis, along with the PCR technique. Among the total of 206 individuals examined, 72 people (35%) were found infected with species using combined parasitological methods. By considering the combined results of parasitological methods as the diagnostic gold standard, the Willis technique had a sensitivity of 91.7% compared with 52.8% for the APC, 40.3% for the HMC, 37.5% for FEAC, and 5.6% for the wet mount technique. The diagnostic specificity of all the parasitological methods was 100%. Furthermore, the PCR method detected spp. DNA in 79 fecal samples (38.3%) with a sensitivity of 97.2% and a specificity of 93.3%. According to the current findings, the Willis method was more sensitive than are the other parasitological methods in the diagnosis of human trichostrongyliasis. However, the PCR assay was more sensitive and more reliable in the detection of human trichostrongyliasis in comparison with the parasitological methods.
人体毛圆线虫病是一种人畜共患疾病，在一些国家的农村地区较为流行。本研究旨在评估各种寄生虫学方法和聚合酶链反应（PCR）在人体毛圆线虫病诊断中的应用。共采集了来自伊朗北部流行地区的 206 例新鲜粪便样本。所有样本均采用常规寄生虫学方法进行检查，包括湿片镜检、甲醛-乙酸乙酯沉淀法（FEAC）、琼脂平板培养（APC）、原田-森木培养（HMC）和 Willis 法，同时还采用了 PCR 技术。在接受检查的 206 名个体中，72 人（35%）通过联合寄生虫学方法被诊断为感染了 种。将寄生虫学方法的联合结果视为诊断金标准，Willis 法的灵敏度为 91.7%，APC 为 52.8%，HMC 为 40.3%，FEAC 为 37.5%，湿片镜检为 5.6%。所有寄生虫学方法的诊断特异性均为 100%。此外，PCR 方法在 79 份粪便样本（38.3%）中检测到了 spp. DNA，灵敏度为 97.2%，特异性为 93.3%。根据目前的研究结果，Willis 法在人体毛圆线虫病的诊断中比其他寄生虫学方法更敏感。然而，与寄生虫学方法相比，PCR 检测法在检测人体毛圆线虫病方面更敏感、更可靠。
