Bogiel Tomasz, Deptuła Aleksander, Kwiecińska-Piróg Joanna, Prażyńska Małgorzata, Mikucka Agnieszka, Gospodarek-Komkowska Eugenia
Department of Microbiology, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, Poland.
Pol J Microbiol. 2017 Dec 4;66(4):427-431. doi: 10.5604/01.3001.0010.6500.
Pseudomonas aeruginosa rods are one of the most commonly isolated microorganisms from clinical specimens, usually responsible for nosocomial infections. Antibiotic-resistant P. aeruginosa strains may present reduced expression of virulence factors. This fact may be caused by appropriate genome management to adapt to changing conditions of the hospital environment. Virulence factors genes may be replaced by those crucial to survive, like antimicrobial resistance genes. The aim of this study was to evaluate, using PCR, the occurrence of exoenzyme S-coding gene (exoS) in two distinct groups of P. aeruginosa strains: 83 multidrug-sensitive (MDS) and 65 multidrug-resistant (MDR) isolates. ExoS gene was noted in 72 (48.7%) of the examined strains: 44 (53.0%) MDS and 28 (43.1%) MDR. The observed differences were not statistically significant (p = 0.1505). P. aeruginosa strains virulence is rather determined by the expression regulation of the possessed genes than the difference in genes frequency amongst strains with different antimicrobial susceptibility patterns.
铜绿假单胞菌杆菌是临床标本中最常分离出的微生物之一，通常引起医院感染。耐抗生素的铜绿假单胞菌菌株可能表现出毒力因子表达降低。这一事实可能是由于适当的基因组管理以适应医院环境不断变化的条件所致。毒力因子基因可能被那些对生存至关重要的基因所取代，如抗微生物耐药基因。本研究的目的是使用聚合酶链反应（PCR）评估两组不同的铜绿假单胞菌菌株中外毒素S编码基因（exoS）的出现情况：83株多重药物敏感（MDS）菌株和65株多重耐药（MDR）菌株。在所检测的菌株中，72株（48.7%）检测到exoS基因：44株（53.0%）MDS菌株和28株（43.1%）MDR菌株。观察到的差异无统计学意义（p = 0.1505）。铜绿假单胞菌菌株的毒力相当程度上取决于所拥有基因的表达调控，而非不同抗微生物药敏模式菌株间基因频率的差异。
