Departamento de Farmacología, Farmacia y Tecnología Farmacéutica, R+D Pharma Group (GI-1645), Facultad de Farmacia and Health Research Institute of Santiago de Compostela (IDIS), Universidade de Santiago de Compostela , E-15782 Santiago de Compostela, Spain.
FEUP-Faculdade de Engenharia, Universidade do Porto, I3S-Instituto de Investigação e Inovação em Saúde, and INEB-Instituto de Engenharia Biomédica , Porto 4200-135, Portugal.
ACS Appl Mater Interfaces. 2018 Jan 31;10(4):3349-3360. doi: 10.1021/acsami.7b17375. Epub 2018 Jan 19.
Advanced porous synthetic scaffolds are particularly suitable for regeneration of damaged tissues, but there is the risk of infections due to the colonization of microorganisms, forming biofilms. Supercritical foaming is an attractive processing method to prepare bone scaffolds, regulating simultaneously the porosity and loading of bioactive compounds without loss of activity. In this work, scaffolds made of poly-ε-caprolactone (50 kDa), containing chitosan and an antimicrobial agent (vancomycin), were processed by supercritical CO foaming for bone regeneration purposes. The obtained scaffolds showed a suitable combination of morphological (porosity, pore size distribution, and interconnectivity), time-dependent in vitro vancomycin release behavior and biological properties (cell viability and proliferation, osteodifferentiation, and tissue-scaffold integration). The scaffolds sustained vancomycin release for more than 2 weeks. Finally, the antimicrobial activity of the scaffolds was tested against Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria after 24 h of incubation with full growth inhibition for S. aureus.
高级多孔合成支架特别适合于受损组织的再生，但由于微生物的定植，形成生物膜，存在感染的风险。超临界发泡是一种有吸引力的制备骨支架的加工方法，可在不损失活性的情况下同时调节多孔性和生物活性化合物的负载。在这项工作中，由聚己内酯（50 kDa）、壳聚糖和一种抗菌剂（万古霉素）制成的支架通过超临界 CO 发泡进行加工，以用于骨再生。所得支架具有合适的形态组合（孔隙率、孔径分布和连通性）、时变的体外万古霉素释放行为和生物学性能（细胞活力和增殖、成骨分化和组织-支架整合）。支架能够持续释放万古霉素超过 2 周。最后，在孵育 24 小时后，支架对革兰氏阳性（金黄色葡萄球菌）和革兰氏阴性（大肠杆菌）细菌的抗菌活性进行了测试，金黄色葡萄球菌的生长完全受到抑制。
