Chinese Academy of Fishery Sciences, Beijing, China.
Key Laboratory of Control of Quality and Safety for Aquatic Products, Ministry of Agriculture, Beijing, China.
J Sci Food Agric. 2018 Jul;98(9):3485-3491. doi: 10.1002/jsfa.8867. Epub 2018 Feb 26.
Methyleugenol is a common phenylpropanoid compound found in many plants and has been widely used as a flavoring agent in people's daily life. In this study, a stable isotope dilution assay-coupled gas chromatography/triple quadrupole mass spectrometry (SIDA-GC/MS/MS) method was developed for the quantitative determination of methyleugenol in food samples. Methyleugenol-D was synthesized and used as an isotope internal standard for the determination of methyleugenol. The QuEChERS (quick, easy, cheap, effective, rugged and safe) method was applied to the clean-up of food sample extracts. Confirmation and quantification were carried out by GC/MS/MS.
The analytical performance of the method was validated. The determination range of methyleugenol was linear from 4 to 500 µg L . Method detection limits for solid food samples, semi-solid food samples and liquid beverages were 50, 50 and 1 µg kg respectively. Satisfactory recoveries in the range 94.29-100.27% were obtained. Intra- and inter-day precision was also validated and the values were all lower than 9%. The method was successfully applied to quantify methyleugenol in different kinds of food samples.
This article describes a new method for the accurate quantification of methyleugenol in food samples based on a stable isotope labeling-assisted GC/MS/MS method. Methyleugenol-D was synthesized and used as an isotope internal standard for the determination of methyleugenol. Excellent results were generated with the method, and the detection sensitivity and accuracy of the method were good. © 2018 Society of Chemical Industry.
甲基丁香酚是一种常见的苯丙烷类化合物，存在于许多植物中，已被广泛用作人们日常生活中的调味剂。在本研究中，建立了一种基于稳定同位素稀释分析（SIDA）-气相色谱/三重四极杆串联质谱（GC/MS/MS）联用的方法，用于食品样品中甲基丁香酚的定量分析。合成了甲基丁香酚-D，并将其用作测定甲基丁香酚的同位素内标。采用 QuEChERS（快速、简单、廉价、有效、耐用和安全）方法对食品样品提取物进行净化。通过 GC/MS/MS 进行确证和定量分析。
对方法的分析性能进行了验证。甲基丁香酚的测定范围在 4 至 500 μg L 之间呈线性。固体食品样品、半固体食品样品和液体饮料的方法检出限分别为 50、50 和 1 μg kg 。在 94.29-100.27%的范围内获得了令人满意的回收率。日内和日间精密度也得到了验证，其值均低于 9%。该方法成功地应用于不同种类食品样品中甲基丁香酚的定量分析。
本文描述了一种基于稳定同位素标记辅助 GC/MS/MS 方法准确测定食品样品中甲基丁香酚的新方法。合成了甲基丁香酚-D，并将其用作测定甲基丁香酚的同位素内标。该方法取得了优异的结果，具有良好的检测灵敏度和准确性。 © 2018 英国化学学会。
