Lopez Patricia, van Sisseren Maarten, De Marco Stefania, Jekel Ad, de Nijs Monique, Mol Hans G J
RIKILT Institute of Food Safety, Wageningen University and Research Centre, Akkermaalsbos 2, 6708 WB Wageningen, The Netherlands.
RIKILT Institute of Food Safety, Wageningen University and Research Centre, Akkermaalsbos 2, 6708 WB Wageningen, The Netherlands.
Food Chem. 2015 May 1;174:407-16. doi: 10.1016/j.foodchem.2014.11.011. Epub 2014 Nov 18.
A straightforward GC-MS method was developed to determine the occurrence of fourteen flavouring compounds in food. It was successfully validated for four generic types of food (liquids, semi-solids, dry solids and fatty solids) in terms of limit of quantification, linearity, selectivity, matrix effects, recovery (53-120%) and repeatability (3-22%). The method was applied to a survey of 61 Dutch food products. The survey was designed to cover all the food commodities for which the EU Regulation 1334/2008 set maximum permitted levels. All samples were compliant with EU legislation. However, the levels of coumarin (0.6-63 mg/kg) may result in an exposure that, in case of children, would exceed the tolerable daily intake (TDI) of 0.1mg/kg bw/day. In addition to coumarin, estragole, methyl-eugenol, (R)-(+)-pulegone and thujone were EU-regulated substances detected in thirty-one of the products. The non-EU regulated alkenylbenzenes, trans-anethole and myristicin, were commonly present in beverages and in herbs-containing products.
开发了一种简单的气相色谱-质谱联用（GC-MS）方法来测定食品中14种调味化合物的含量。该方法在定量限、线性、选择性、基质效应、回收率（53%-120%）和重复性（3%-22%）方面成功地针对四种通用类型的食品（液体、半固体、干固体和脂肪固体）进行了验证。该方法应用于对61种荷兰食品的调查。该调查旨在涵盖欧盟法规1334/2008规定了最大允许含量的所有食品商品。所有样品均符合欧盟法规。然而，香豆素的含量（0.6-63毫克/千克）可能导致的暴露量，对于儿童而言，可能会超过每日可耐受摄入量（TDI）0.1毫克/千克体重/天。除香豆素外，在31种产品中还检测到了欧盟规定的物质，如草蒿脑、甲基丁香酚、（R）-（+）-胡薄荷酮和侧柏酮。非欧盟规定的链烯基苯、反式茴香脑和肉豆蔻醚通常存在于饮料和含草药产品中。
