College of Animal Science and Technology, Zhejiang A & F University, Lin'an, China.
College of Animal Sciences, Zhejiang University, Hangzhou, China.
J Anim Physiol Anim Nutr (Berl). 2018 Apr;102(2):e909-e917. doi: 10.1111/jpn.12856. Epub 2018 Jan 3.
Studies have found that a dietary supplement of Bacillus amyloliquefaciens improved the growth performance, increased the nutrient digestibility of hosts and modulated the intestinal microflora. A total of 360 1-day-old Ross broilers were randomly divided into three treatments: a control group with a basal diet, an antibiotic group with a basal diet and added colistin sulphate, and a probiotics group with a basal diet and added Bacillus amyloliquefaciens. The HiSeq high-throughput sequencing analysis of 16S rRNA was used to investigate the differences in birds' caecal microflora, and metabolomics was used to analyse changes in caecal metabolites. Results showed that the supplementation of Bacillus amyloliquefaciens significantly improved the BW and ADG compared with the control birds. Results of sequencing indicated that (i) 645, 670, 596 unique operational taxonomic units (OTUs) were found in birds supplemented with Bacillus amyloliquefaciens on day 7, 21 and 42, separately, (ii) due to the diversity and relative abundance of the birds' caecal microflora, the OTUs of the caecal microflora clustered according to age and treatment, except on day 42, (iii) among the six predominate families (Ruminococcaceae, Lachnospiraceae, Enterobacteriaceae, Erysipelotrichaceae, Lactobacillaceae and Rikenellaceae), the supplementation of Bacillus amyloliquefaciens significantly increased Enterobacteriaceae on day 42, (iv) Bacillus amyloliquefaciens increased the relative abundance of Faecalibacterium and Ruminococcus on day 21, increased the Faecalibacterium and Blautia and decreased the Ruminococcus on day 42. The metabolomics of caecal metabolites showed that the dietary Bacillus amyloliquefaciens changed the caecal metabolites involved of amino acid metabolism and glyceride metabolism, and the antibiotics changed the caecal metabolites that were related to carbohydrates and amino acid metabolism on day 21.
研究发现，地衣芽孢杆菌的膳食补充剂改善了生长性能，提高了宿主的营养消化率，并调节了肠道微生物群。将 360 只 1 日龄罗斯肉鸡随机分为三组：基础日粮对照组、基础日粮加硫酸粘菌素抗生素组和基础日粮加地衣芽孢杆菌益生菌组。使用 16S rRNA 的 HiSeq 高通量测序分析来研究鸟类盲肠微生物群的差异，并使用代谢组学分析盲肠代谢物的变化。结果表明，与对照组相比，地衣芽孢杆菌的补充显著提高了 BW 和 ADG。测序结果表明：（i）在第 7、21 和 42 天，分别在补充地衣芽孢杆菌的鸟类中发现了 645、670 和 596 个独特的操作分类单元（OTUs）；（ii）由于鸟类盲肠微生物群的多样性和相对丰度，盲肠微生物群的 OTUs 根据年龄和处理进行聚类，除了第 42 天；（iii）在六个主要科（瘤胃球菌科、毛螺菌科、肠杆菌科、埃希氏菌科、乳杆菌科和理研菌科）中，地衣芽孢杆菌的补充显著增加了第 42 天的肠杆菌科；（iv）地衣芽孢杆菌在第 21 天增加了粪杆菌和真杆菌的相对丰度，在第 42 天增加了粪杆菌和布劳特氏菌，减少了真杆菌。盲肠代谢物的代谢组学表明，膳食地衣芽孢杆菌改变了涉及氨基酸代谢和甘油酯代谢的盲肠代谢物，抗生素改变了与碳水化合物和氨基酸代谢相关的盲肠代谢物在第 21 天。
