Han Haixia, Fu Li, Wang Jie, Sun Yan, Cao Dingguo, Lei Qiuxia, Zhou Yan, Li Fuwei, Liu Wei, Li Dapeng, Hao Dan, Liu Jie
Poultry Research Institute, Shandong Academy of Agricultural Sciences, Ji'nan, China.
Main Animal Breeding Key Laboratory of Shandong Province, Ji'nan, China.
Front Microbiol. 2025 Jun 18;16:1608076. doi: 10.3389/fmicb.2025.1608076. eCollection 2025.
This study aimed to investigate the effects of () supplementation on growth performance, carcass traits, antioxidant capacity, and cecal microbiota in broilers. A total of 320 one-day-old YS909 male broilers were randomly assigned to four dietary groups: control (CON), low-dose (LBL, 100 mg/kg), medium-dose (MBL, 300 mg/kg), and high-dose (HBL, 500 mg/kg) supplementation ( = 8 replicates/group, 10 chicks/replicate). Growth performance, carcass traits, antioxidant capacity, and cecal microbiota/metabolites (MBL vs. CON) were analyzed. Dietary supplementation with significantly decreased the feed intake / weight gain (F/G) in LBL (22-42 d and 1-42 d), MBL (22-42 d) and HBL (22-42 d) groups. Both MBL and HBL groups showed higher semi-eviscerated percentages than the control. The MBL group had a significantly increased eviscerated percentage. The LBL group had a significantly increased breast muscle percentage. Specifically, the HBL group exhibited a notable increase in muscle C18:3n3 content, and a significant decrease in muscle C18:1n9t and C20:3n3 content. The LBL group saw significant reductions in the proportion of C18:1n9t and C20:1. Additionally, the MBL group experienced significant decreases in the proportions of C18:3n3, C20:3n3 and C20:4n6. Dietary supplementation with significantly enhanced the oxidative stress resistance of serum by decreasing malondialdehyde (MDA) levels and increasing glutathione peroxidase (GSH-PX) and total antioxidant capacity (T-AOC). 16S ribosomal DNA and metabolome sequencing of cecum contents was conducted for the MBL and CON groups. This analysis demonstrated significant increases in α-diversity indices in the MBL group. There was an increased relative abundance of and a decreased relative abundance of and in the MBL group. In addition, the shifts of the cecal microbial community lead to the alteration of metabolites of the cecum including amino acid and lipid. In conclusion, dietary supplementation with medium-dose enhanced broiler carcass traits and antioxidant status by modulating cecal microbiota and metabolites, demonstrating its potential as an effective feed additive.
本研究旨在探讨（）添加物对肉鸡生长性能、胴体性状、抗氧化能力和盲肠微生物群的影响。总共320只1日龄YS909雄性肉鸡被随机分为四个日粮组：对照组（CON）、低剂量组（LBL，100毫克/千克）、中剂量组（MBL，300毫克/千克）和高剂量组（HBL，500毫克/千克）添加物组（每组8个重复，每个重复10只雏鸡）。分析了生长性能、胴体性状、抗氧化能力和盲肠微生物群/代谢物（MBL与CON）。日粮添加（）显著降低了LBL组（22 - 42日龄和1 - 42日龄）、MBL组（22 - 42日龄）和HBL组（22 - 42日龄）的料重比（F/G）。MBL组和HBL组的半净膛率均高于对照组。MBL组的全净膛率显著提高。LBL组的胸肌率显著提高。具体而言，HBL组肌肉中C18:3n3含量显著增加，肌肉中C18:1n9t和C20:3n3含量显著降低。LBL组C18:1n9t和C20:1的比例显著降低。此外，MBL组C18:3n3、C20:3n3和C20:4n6的比例显著降低。日粮添加（）通过降低丙二醛（MDA）水平、增加谷胱甘肽过氧化物酶（GSH - PX）和总抗氧化能力（T - AOC）显著增强了血清的抗氧化应激能力。对MBL组和CON组的盲肠内容物进行了16S核糖体DNA和代谢组测序。该分析表明MBL组的α - 多样性指数显著增加。MBL组中（某菌属）相对丰度增加，（另外两个菌属）相对丰度降低。此外，盲肠微生物群落的变化导致盲肠代谢物（包括氨基酸和脂质）的改变。总之，日粮中添加中剂量（）通过调节盲肠微生物群和代谢物提高了肉鸡的胴体性状和抗氧化状态，证明了其作为有效饲料添加剂的潜力。
