Department of Cytology, Institute of Zoology, Faculty of Biology, University of Warsaw, Poland.
Laboratory of Cytometry, Nencki Institute of Experimental Biology.
Stem Cells. 2018 May;36(5):655-670. doi: 10.1002/stem.2772. Epub 2018 Jan 22.
MicroRNAs (miRNAs) are known regulators of various cellular processes, including pluripotency and differentiation of embryonic stem cells (ESCs). We analyzed differentiation of two ESC lines-D3 and B8, and observed significant differences in the expression of miRNAs and genes involved in pluripotency and differentiation. We also examined if transient miRNA overexpression could serve as a sufficient impulse modulating differentiation of mouse ESCs. ESCs were transfected with miRNA Mimics and differentiated in embryoid bodies and embryoid body outgrowths. miRNAs involved in differentiation of mesodermal lineages, such as miR145 and miR181, as well as miRNAs regulating myogenesis (MyomiRs)-miR1, miR133a, miR133b, and miR206 were tested. Using such approach, we proved that transient overexpression of molecules selected by us modulated differentiation of mouse ESCs. Increase in miR145 levels upregulated Pax3, Pax7, Myod1, Myog, and MyHC2, while miR181 triggered the expression of such crucial myogenic factors as Myf5 and MyHC2. As a result, the ability of ESCs to initiate myogenic differentiation and form myotubes was enhanced. Premature expression of MyomiRs had, however, an adverse effect on myogenic differentiation of ESCs. Stem Cells 2018;36:655-670.
微小 RNA（miRNAs）是各种细胞过程的已知调节剂，包括胚胎干细胞（ESCs）的多能性和分化。我们分析了两个 ESC 系 D3 和 B8 的分化，观察到 miRNA 和参与多能性和分化的基因的表达有显著差异。我们还研究了瞬时 miRNA 过表达是否可以作为调节小鼠 ESC 分化的充分冲动。将 miRNA 模拟物转染到 ESCs 中，并在胚状体和胚状体外生体中进行分化。测试了涉及中胚层谱系分化的 miRNA，如 miR145 和 miR181，以及调节肌发生的 miRNA（MyomiRs）-miR1、miR133a、miR133b 和 miR206。通过这种方法，我们证明了我们选择的分子的瞬时过表达调节了小鼠 ESCs 的分化。miR145 水平的增加上调了 Pax3、Pax7、Myod1、Myog 和 MyHC2，而 miR181 则触发了 Myf5 和 MyHC2 等关键肌生成因子的表达。因此，增强了 ESCs 启动肌生成分化并形成肌管的能力。然而，MyomiRs 的过早表达对 ESCs 的肌生成分化有不利影响。干细胞 2018;36:655-670。
