Department of Cytology, Institute of Developmental Biology and Biomedical Sciences, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland.
Cells. 2021 Aug 26;10(9):2205. doi: 10.3390/cells10092205.
PAX7 transcription factor plays a crucial role in embryonic myogenesis and in adult muscles in which it secures proper function of satellite cells, including regulation of their self renewal. PAX7 downregulation is necessary for the myogenic differentiation of satellite cells induced after muscle damage, what is prerequisite step for regeneration. Using differentiating pluripotent stem cells we documented that the absence of functional PAX7 facilitates proliferation. Such action is executed by the modulation of the expression of two proteins involved in the DNA methylation, i.e., and . Increase in expression led to the downregulation of the CDK inhibitors and facilitated cell cycle progression. Changes in expression, on the other hand, differently impacted proliferation/differentiation balance, depending on the experimental model used.
PAX7 转录因子在胚胎肌发生和成年肌肉中起着至关重要的作用，它确保了卫星细胞的正常功能，包括调节其自我更新。PAX7 的下调对于肌肉损伤后诱导的卫星细胞的成肌分化是必要的，这是再生的前提步骤。使用分化的多能干细胞，我们记录到功能性 PAX7 的缺失促进了增殖。这种作用是通过调节两种参与 DNA 甲基化的蛋白质的表达来执行的，即 和 。表达增加导致 CDK 抑制剂下调，并促进细胞周期进程。另一方面，表达的变化根据所使用的实验模型，对增殖/分化平衡产生不同的影响。
