Yang Bi-Cheng, Wan Xi-di, Yang Xiao, Li Yun-Jun, Zhang Zi-Yu, Wan Xiao-Ju, Luo Yong, Deng Wei, Wang Feng, Huang Ou-Ping
Jiangxi Provincial Maternal and Child Health Hospital, Nanchang, Jiangxi, 330006, China.
J Mass Spectrom. 2018 Mar;53(3):234-239. doi: 10.1002/jms.4057.
The determination of pesticide residues is an indispensable task in controlling food safety and environment protection. Carbendazim is one of the extensive uses of pesticides in the agricultural industry. In this study, a simple method utilizing syringe filter has been applied as electrospray ionization emitter for mass spectrometric identification and quantification of carbendazim in complex matrices including soil, natural water, and fruit juice samples, which contain many insoluble materials. With online syringe filter of the complex samples, most of insoluble materials such as soil were excluded in spray ionization process due to the filter effect, and analytes were subsequently sprayed out from syringe needle for mass spectrometric detection. The pore sizes of filters and diameters of syringe needles also were investigated. The analytical performances, including the linear range (1-200 ng·mL ), limit of detection (0.2-0.6 ng·mL , S/N > 3), limit of quantitation (3.5-8.6 ng·mL , S/N > 10), reproducibility (6.4%-12.5%, n = 6), and recoveries (72.1%-91.0%, n = 6) were well acceptable for direct analysis of raw samples. Matrix effect for detection of carbendazim in soil samples also was experimentally investigated. This study demonstrated that syringe filter needle coupled with electrospray ionization mass spectrometry is a simple, efficient, and sensitive method for detection of pesticide residues in water, soil, and fruit juice for risk assessment.
农药残留的测定是食品安全控制和环境保护中不可或缺的任务。多菌灵是农业行业中广泛使用的农药之一。在本研究中，一种利用注射器过滤器的简单方法被用作电喷雾电离发射器，用于对包括土壤、天然水和果汁样品在内的复杂基质中的多菌灵进行质谱鉴定和定量，这些样品含有许多不溶性物质。通过对复杂样品进行在线注射器过滤，由于过滤作用，大多数不溶性物质（如土壤）在喷雾电离过程中被排除，随后分析物从注射器针头喷出进行质谱检测。还研究了过滤器的孔径和注射器针头的直径。该分析方法的性能，包括线性范围（1 - 200 ng·mL）、检测限（0.2 - 0.6 ng·mL，S/N > 3）、定量限（3.5 - 8.6 ng·mL，S/N > 10）、重现性（6.4% - 12.5%，n = 6）和回收率（72.1% - 91.0%，n = 6）对于直接分析原始样品来说是完全可以接受的。还通过实验研究了土壤样品中多菌灵检测的基质效应。本研究表明，注射器过滤针头与电喷雾电离质谱联用是一种用于水、土壤和果汁中农药残留检测以进行风险评估的简单、高效且灵敏方法。
