Laboratory for Medical Microbiology, Mölbis, Germany.
Institute for Bacteriology and Mycology, Veterinary Faculty at the University of Leipzig, Leipzig, Germany.
J Dtsch Dermatol Ges. 2018 Jan;16(1):21-32. doi: 10.1111/ddg.13408.
Formerly only referred to as a subspecies (T. mentagrophytes var. quinckeanum), T. quinckeanum once again constitutes a distinct species according to the updated taxonomy of dermatophytes.
During routine diagnostic tests conducted at the Mycology Laboratory, Mölbis, Germany, between 11/2013 to 1/2017 (three years and three months), all specimens sent in were examined for T. quinckeanum. Molecular biology methods employed included: 1) DNA hybridization (PCR ELISA), 2) gene sequencing of the ITS region and TEF-1α, and 3) in some cases, MALDI-TOF mass spectrometry.
Overall, 62 strains of T. quinckeanum were found. Sixty-eight percent of patients were female; 43 % were children and adolescents (≤ 19 years of age). Cats were a frequent source of infection. Sequencing of all 62 strains revealed a concordance of 100 % with T. quinckeanum sequences contained in the NCBI database. Isolates analyzed by MALDI-TOF mass spectrometry showed specific spectra.
In Germany, the zoophilic dermatophyte T. quinckeanum currently appears to be more prevalent than expected. T. quinckeanum strains were isolated from children and adults with dermatomycosis and tinea capitis. Sources of infection with T. quinckeanum include small rodents (mice), horses, and - remarkably commonly - cats. Given that unequivocal morphological identification of this dermatophyte is not always possible, molecular methods have to be employed in the diagnosis.
根据更新的皮肤真菌分类学，原先仅被称为亚种（T. mentagrophytes var. quinckeanum）的须癣毛癣菌（T. quinckeanum）再次构成了一个独特的物种。
在德国莫尔比茨的真菌学实验室于 2013 年 11 月至 2017 年 1 月（三年零三个月）进行的常规诊断检测期间，所有送检标本均被检测是否存在须癣毛癣菌。采用的分子生物学方法包括：1）DNA 杂交（PCR ELISA），2）ITS 区和 TEF-1α 基因测序，以及 3）在某些情况下采用 MALDI-TOF 质谱法。
总体而言，发现了 62 株须癣毛癣菌。68%的患者为女性；43%为儿童和青少年（≤19 岁）。猫是常见的感染源。对所有 62 株菌株进行测序，与 NCBI 数据库中包含的须癣毛癣菌序列完全一致，一致性为 100%。通过 MALDI-TOF 质谱分析的分离株显示出特异性的光谱。
在德国，亲动物性皮肤真菌须癣毛癣菌目前似乎比预期更为普遍。须癣毛癣菌菌株从患有皮肤癣菌病和头癣的儿童和成人中分离出来。感染须癣毛癣菌的来源包括小型啮齿动物（老鼠）、马，以及——非常常见——猫。鉴于这种皮肤真菌的形态学鉴定并不总是明确，因此必须在诊断中采用分子方法。
