Tworkoski Ellen, Glucksberg Matthew R, Johnson Mark
Department of Biomedical Engineering, Northwestern University, Evanston, Illinois, United States of America.
Department of Mechanical Engineering, Northwestern University, Evanston, Illinois, United States of America.
PLoS One. 2018 Jan 9;13(1):e0189890. doi: 10.1371/journal.pone.0189890. eCollection 2018.
Changes in hydrostatic pressure, at levels as low as 10 mm Hg, have been reported in some studies to alter cell function in vitro; however, other studies have found no detectable changes using similar methodologies. We here investigate the hypothesis that the rate of depressurization, rather than elevated hydrostatic pressure itself, may be responsible for these reported changes. Hydrostatic pressure (100 mm Hg above atmospheric pressure) was applied to bovine aortic endothelial cells (BAECs) and PC12 neuronal cells using pressurized gas for periods ranging from 3 hours to 9 days, and then the system was either slowly (30 minutes) or rapidly (5 seconds) depressurized. Cell viability, apoptosis, proliferation, and F-actin distribution were then assayed. Our results did not show significant differences between rapidly and slowly depressurized cells that would explain differences previously reported in the literature. Moreover, we found no detectable effect of elevated hydrostatic pressure (with slow depressurization) on any measured variables. Our results do not confirm the findings of other groups that modest increases in hydrostatic pressure affect cell function, but we are not able to explain their findings.
一些研究报告称，低至10毫米汞柱的流体静压变化会在体外改变细胞功能；然而，其他研究使用类似方法却未发现可检测到的变化。我们在此研究一个假说，即减压速率而非流体静压升高本身可能是这些报告中变化的原因。使用加压气体对牛主动脉内皮细胞（BAECs）和PC12神经元细胞施加流体静压（高于大气压100毫米汞柱），持续时间从3小时到9天不等，然后系统以缓慢（约30分钟）或快速（约5秒）的方式减压。接着测定细胞活力、凋亡、增殖和F-肌动蛋白分布。我们的结果并未显示快速减压和缓慢减压细胞之间存在显著差异，而这种差异本可解释先前文献中报道的差异。此外，我们未发现流体静压升高（伴随缓慢减压）对任何测量变量有可检测到的影响。我们的结果并未证实其他研究小组的发现，即适度升高流体静压会影响细胞功能，但我们无法解释他们的发现。
