Laboratorio de Virología Molecular, CENUR Litoral Norte, Centro Universitario de Salto, Universidad de la República, Salto, Uruguay.
Plataforma de Investigación en Salud Animal, Instituto Nacional de Investigación Agropecuaria (INIA), La Estanzuela, Colonia, Uruguay.
J Appl Microbiol. 2018 Mar;124(3):922-929. doi: 10.1111/jam.13691. Epub 2018 Feb 7.
To analyse group A rotavirus (RVA) environmental contamination in waters used for calves' consumption and to assess viral viability in dairy farm water sources.
We analysed 202 samples of water used for calves' consumption and RVA was detected by RT-qPCR in 35·1% (95% CI: 28·9-42·0%). A marked pattern of seasonality was observed with higher frequency of detection in colder than warmer months (P = 0·002). There was no association between viral load and season or between the number of milking cows in the herd and the detection of RVA in the farm. The viability of the RVA particles detected was confirmed by isolation of RVA in cell culture from 5 of 10 water samples. Furthermore, an RVA waterborne outbreak of neonatal calf diarrhoea was described.
We demonstrate that RVA is frequent in dairy farm waters, and that the virus is infectious and capable of generating a diarrhoea outbreak.
Neonatal diarrhoea syndrome leads to economic losses to the livestock industry worldwide. To determine transmission routes is essential to take action in this regard and reduce the impact that this syndrome has for the livestock production. The results obtained in this work alert the dairy industry and highlight that mitigation strategies are crucial to improve the microbiological quality of this water.
分析用于犊牛饮用的水中 A 组轮状病毒（RVA）的环境污染情况，并评估奶牛场水源中病毒的存活能力。
我们分析了 202 份用于犊牛饮用的水样本，通过 RT-qPCR 在 35.1%（95%置信区间：28.9-42.0%）的样本中检测到了 RVA。检测到的病毒呈明显的季节性模式，在较冷月份的检测频率高于较暖月份（P=0.002）。病毒载量与季节之间、牛群中奶牛数量与农场中 RVA 的检测之间均无相关性。通过从 10 个水样中的 5 个水样中分离出 RVA，证实了所检测到的 RVA 颗粒的存活能力。此外，还描述了一起由 RVA 引起的犊牛腹泻的水源暴发事件。
我们证明 RVA 在奶牛场水中很常见，并且该病毒具有传染性，能够引发腹泻暴发。
新生犊牛腹泻综合征导致全球畜牧业遭受经济损失。确定传播途径对于采取行动和减少该综合征对畜牧业生产的影响至关重要。本工作的结果提醒了奶制品行业，突出了缓解策略对于改善该水的微生物质量至关重要。
