Instituto de Virología, CICV y A INTA, Buenos Aires, Argentina.
Infect Genet Evol. 2013 Aug;18:18-30. doi: 10.1016/j.meegid.2013.04.023. Epub 2013 Apr 26.
Group A rotavirus (RVA) is one of the main causes of neonatal calf diarrhea worldwide. RVA strains affecting Argentinean cattle mainly possess combinations of the G6, G10, P[5] and P[11] genotypes. To determine RVA diversity among Argentinean cattle, representative bovine RVA strains detected in diarrheic calves were selected from a survey conducted during 1997-2009. The survey covered the main livestock regions of the country from dairy and beef herds. Different phylogenetic approaches were used to investigate the genetic evolution of RVA strains belonging to the prevalent genotypes. The nucleotide phylogenetic tree showed that all genotypes studied could be divided into several lineages. Argentinean bovine RVA strains were distributed across multiple lineages and most of them were distinct from the lineage containing the vaccine strains. Only the aminoacid phylogenetic tree of G6 RVA strains maintained the same lineages as observed at the nucleotide level, whereas a different clustering pattern was observed for the aminoacid phylogenetic trees of G10, P[5] and P[11] suggesting that the strains are more closely related at the aminoacid level than G6 strains. Association between P[5] and G6(IV), prevalent in beef herd, and between P[11] and G6(III) or G10 (VI and V), prevalent in dairy herds, were found. In addition, Argentinean G6(III), G10, P[5] and P[11] bovine RVA strains grouped together with human strains, highlighting their potential for zoonotic transmission. Phylogenetic studies of RVA circulating in animals raised for consumption and in close contact with humans, such as cattle, contribute to a better understanding of the epidemiology of the RVA infection and evolution.
A 组轮状病毒（RVA）是全球导致新生犊牛腹泻的主要原因之一。在阿根廷流行的 RVA 毒株主要具有 G6、G10、P[5]和 P[11]基因型的组合。为了确定 RVA 在阿根廷牛群中的多样性，从 1997 年至 2009 年进行的一项调查中选择了在腹泻犊牛中检测到的代表性牛 RVA 株。该调查涵盖了该国主要的畜牧业地区，包括奶牛和肉牛群。使用不同的系统发育方法来研究属于流行基因型的 RVA 株的遗传进化。核苷酸系统发育树表明，所有研究的基因型都可以分为几个谱系。阿根廷牛 RVA 株分布在多个谱系中，大多数与包含疫苗株的谱系不同。只有 G6 RVA 株的氨基酸系统发育树在核苷酸水平上保持了相同的谱系，而 G10、P[5]和 P[11]的氨基酸系统发育树则观察到不同的聚类模式，表明这些菌株在氨基酸水平上比 G6 菌株更为密切相关。在肉牛群中流行的 P[5]和 G6(IV)以及在奶牛群中流行的 P[11]和 G6(III)或 G10 (VI 和 V)之间存在关联。此外，阿根廷的 G6(III)、G10、P[5]和 P[11]牛 RVA 株与人类株聚集在一起，突出了它们发生人畜共患传播的潜力。对用于消费且与人类密切接触的动物（如牛）中循环的 RVA 的系统发育研究有助于更好地了解 RVA 感染和进化的流行病学。
