酵母芽殖期具有最小的内膜系统。
Budding Yeast Has a Minimal Endomembrane System.
机构信息
Department of Molecular Genetics and Cell Biology, University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA.
Department of Molecular Genetics and Cell Biology, University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA.
出版信息
Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi: 10.1016/j.devcel.2017.12.014.
The endomembrane system consists of the secretory and endocytic pathways, which communicate by transport to and from the trans-Golgi network (TGN). In mammalian cells, the endocytic pathway includes early, late, and recycling endosomes. In budding yeast, different types of endosomes have been described, but the organization of the endocytic pathway has remained unclear. We performed a spatial and temporal analysis of yeast endosomal markers and endocytic cargoes. Our results indicate that the yeast TGN also serves as an early and recycling endosome. In addition, as previously described, yeast contains a late or prevacuolar endosome (PVE). Endocytic cargoes localize to the TGN shortly after internalization, and manipulations that perturb export from the TGN can slow the passage of endocytic cargoes to the PVE. Yeast apparently lacks a distinct early endosome. Thus, yeast has a simple endocytic pathway that may reflect the ancestral organization of the endomembrane system.
内质网系统包括分泌途径和内吞途径，它们通过运输在高尔基体网络（TGN）之间进行交流。在哺乳动物细胞中，内吞途径包括早期内体、晚期内体和再循环内体。在出芽酵母中，已经描述了不同类型的内体，但内吞途径的组织仍然不清楚。我们对酵母内体标记物和内吞货物进行了时空分析。我们的结果表明，酵母 TGN 也充当早期和再循环内体。此外，如前所述，酵母含有晚期或前液泡内体（PVE）。内吞货物在内化后不久就定位到 TGN，并且干扰从 TGN 输出的操作可以减缓内吞货物向 PVE 的传递。酵母显然缺乏明显的早期内体。因此，酵母具有简单的内吞途径，这可能反映了内质网系统的祖先组织。
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