酿酒酵母中的高通量DNA修复监测表明，单链断裂和双链断裂诱导染色质重排。
High-throughput DNA repair monitoring in Saccharomyces cerevisiae suggests SSB- and DSB-induced chromatin reconfiguration.
作者信息
Shalev Ezra Yael, Saguy Alon, Levin Gaia, Weiss Lucien E, Alalouf Onit, Shechtman Yoav
机构信息
Faculty of Biomedical Engineering, Technion - Israel Institute of Technology, Haifa, 3200003, Israel.
Department of Engineering Physics, Polytechnique Montréal, Montreal, QC, H3T 1J4, Canada.
出版信息
Sci Rep. 2025 Sep 2;15(1):32302. doi: 10.1038/s41598-025-17538-5.
UNLABELLED
DNA repair is critical for cellular function and genomic stability across organisms. Yeast mating-type switching serves as an established model for studying DNA break repair and chromosome dynamics. However, real-time tracking of mating-type switching in live cells remains challenging due to resolution limitations of existing techniques. Here, we use high-throughput methods, including three-dimensional imaging, to follow the dynamics of DNA damage and repair and to quantify mating-type switching occurrences at the single live cell level, with unprecedented resolution. We reveal chromosome reconfiguration for both single- and double-strand breaks following switching induction. Our findings provide new observation of the correlation between chromosome folding and single-strand breaks.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1038/s41598-025-17538-5.
未标记
DNA修复对于所有生物体的细胞功能和基因组稳定性至关重要。酵母交配型转换是研究DNA断裂修复和染色体动态变化的既定模型。然而，由于现有技术的分辨率限制，实时跟踪活细胞中的交配型转换仍然具有挑战性。在这里，我们使用包括三维成像在内的高通量方法，以前所未有的分辨率跟踪DNA损伤和修复的动态变化，并在单个活细胞水平上量化交配型转换的发生情况。我们揭示了转换诱导后单链和双链断裂的染色体重新配置。我们的发现为染色体折叠与单链断裂之间的相关性提供了新的观察结果。
补充信息
在线版本包含可在10.1038/s41598-025-17538-5获取的补充材料。
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