Jerzy Haber Institute of Catalysis and Surface Chemistry Polish Academy of Science, Niezapominajek 8, 30-239 Cracow, Poland.
Jerzy Haber Institute of Catalysis and Surface Chemistry Polish Academy of Science, Niezapominajek 8, 30-239 Cracow, Poland.
J Colloid Interface Sci. 2018 Mar 15;514:769-790. doi: 10.1016/j.jcis.2017.11.083. Epub 2017 Dec 19.
Adsorption of human serum albumin (HSA), recombinant HSA (rHSA) and the albumin dimer (dHSA) at solid/electrolyte interfaces is reviewed with the emphasis put on quantitative analysis of this process. Initially, various physicochemical data characterizing bulk properties of albumin molecules are discussed such as electrophoretic mobility, electrokinetic charge, zeta potential and diffusion coefficient. Adsorption kinetics of HSA, rHSA and dHSA at mica derived from AFM, streaming potential and XPS measurements is analyzed. Maximum coverages of irreversibly adsorbed molecules under various ionic strengths and pHs are quantitatively interpreted in terms of the random sequential adsorption model. Thorough acid-basic characteristic of albumin monolayers of well-controlled coverage are also presented. The results derived from the colloid deposition method that unveil albumin molecule orientation and charge distribution are discussed and interpreted in terms of the random site theory. Subsequently, adsorption of albumins at negatively and positively charged polymeric microparticles studied by the electrokinetic and the AFM aided concentration depletion methods is analyzed. These results are theoretically interpreted by applying the bead model of HSA and dHSA molecules. Orientation of adsorbed molecules and the stability of albumin monolayers in respect to pH cyclic changes are discussed. A universal, electrostatic interaction driven, mechanism of albumin adsorption at macroscopic surfaces and polymer microparticles is confirmed.
在固/电解质界面上，人血清白蛋白（HSA）、重组 HSA（rHSA）和白蛋白二聚体（dHSA）的吸附作用受到了关注，重点是对这一过程进行定量分析。首先，讨论了各种物理化学数据，这些数据描述了白蛋白分子的体相特性，如电泳迁移率、动电电荷、zeta 电位和扩散系数。从原子力显微镜（AFM）、流动电势和 XPS 测量中分析了 HSA、rHSA 和 dHSA 在云母上的吸附动力学。根据随机顺序吸附模型，定量解释了在不同离子强度和 pH 值下，不可逆吸附分子的最大覆盖率。还呈现了具有良好控制覆盖率的白蛋白单层的透彻的酸碱特性。根据胶体沉积方法得出的结果揭示了白蛋白分子的取向和电荷分布，并根据随机位理论进行了讨论和解释。随后，通过电动和 AFM 辅助浓度耗竭方法研究了负电荷和正电荷聚合物微球上的白蛋白吸附。通过应用 HSA 和 dHSA 分子的珠模型对这些结果进行了理论解释。讨论了吸附分子的取向和白蛋白单层在 pH 循环变化方面的稳定性。证实了在宏观表面和聚合物微球上，白蛋白吸附的普遍的静电相互作用驱动机制。
