Salomão Roberta Garcia, de Carvalho Luciana Martins, Izumi Clarice, Czernisz Érika Silva, Rosa José César, Antonini Sonir Roberto Rauber, Bueno Ana Carolina, Almada Maria Olímpia Ribeiro do Vale, Coelho-Landell Carolina de Almeida, Jordão Alceu Afonso, Ferriani Virgínia Paes Leme, Monteiro Jacqueline Pontes
Department of Pediatrics, Ribeirão Preto Medical School, University of São Paulo, Av. Bandeirantes, 3900, 14049-900, Ribeirão Preto, São Paulo, Brasil.
Protein Chemistry Center, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brasil.
Pediatr Rheumatol Online J. 2018 Jan 9;16(1):4. doi: 10.1186/s12969-017-0220-y.
Childhood-onset systemic lupus erythematosus (c-SLE) is a chronic autoimmune disease which increases cardiovascular risk factors (CRF) such as elevated homocysteine, TNF-α, and hs-C reactive protein.
We evaluated BMI, waist circumference (WC), 24-h recalls, SLEDAI-2 K, SLICC/ACR-DI, serum levels of homocysteine, folate, TNF-α, hs-C reactive protein, lipid profile, proteomic data, and duration of corticosteroid therapy in 19 c-SLE and 38 healthy volunteers. Physiological and anthropometric variables of c-SLE and healthy controls were compared by ANCOVA. k-cluster was used to separate c-SLE into two different groups with the best and the worst metabolic profile according to previous analysis showing some metabolites that were statistically different from controls, such as homocysteine, TNF-α, hs-CRP and folate levels. These two clusters were again compared with the control group regarding nutritional parameters, lipid profile and also proteomic data.
Individuals with c-SLE presented higher BMI, WC, homocysteine, triglycerides, TNF-α, hs-CRP and lower folate levels when compared to controls. We found 10 proteins whose relative abundances were statistically different between control group and lupus clusters with the best (LCBMP) and the worst metabolic profile (LCWMP). A significant positive correlation was found between TNF-α and triglycerides and between hs-CRP and duration of corticosteroid therapy.
Cardiovascular disease (CVD) risk parameters were worse in c-SLE. A less protective CVD proteomic profile was found in LCWMP.
儿童期起病的系统性红斑狼疮（c-SLE）是一种慢性自身免疫性疾病，会增加心血管危险因素（CRF），如高同型半胱氨酸、肿瘤坏死因子-α（TNF-α）和超敏C反应蛋白（hs-CRP）水平升高。
我们评估了19例c-SLE患者和38名健康志愿者的体重指数（BMI）、腰围（WC）、24小时饮食回顾、系统性红斑狼疮疾病活动指数2000（SLEDAI-2K）、系统性红斑狼疮国际协作临床/美国风湿病学会损伤指数（SLICC/ACR-DI）、同型半胱氨酸、叶酸、TNF-α、hs-CRP的血清水平、血脂谱、蛋白质组学数据以及皮质类固醇治疗的持续时间。通过协方差分析比较c-SLE患者和健康对照的生理和人体测量学变量。根据先前分析显示一些代谢物与对照组有统计学差异，如同型半胱氨酸、TNF-α、hs-CRP和叶酸水平，使用k聚类将c-SLE分为代谢状况最佳和最差的两个不同组。再次将这两个聚类与对照组在营养参数、血脂谱以及蛋白质组学数据方面进行比较。
与对照组相比，c-SLE患者的BMI、WC、同型半胱氨酸、甘油三酯、TNF-α、hs-CRP水平更高，叶酸水平更低。我们发现10种蛋白质的相对丰度在对照组与代谢状况最佳（LCBMP）和最差（LCWMP）的狼疮聚类之间存在统计学差异。TNF-α与甘油三酯之间以及hs-CRP与皮质类固醇治疗持续时间之间存在显著正相关。
c-SLE患者的心血管疾病（CVD）风险参数更差。在LCWMP中发现了保护性较差的CVD蛋白质组学特征。
