Mastrangelo Salvatore, Biscarini Filippo, Auzino Barbara, Ragatzu Marco, Spaterna Andrea, Ciampolini Roberta
Dipartimento di Scienze Agrarie e Forestali, Università di Palermo, Palermo, Italy.
CNR-IBBA, Via Bassini 15, 20133, Milano, Italy.
BMC Res Notes. 2018 Jan 9;11(1):13. doi: 10.1186/s13104-017-3112-9.
Braque Français, type Pyrénées is a French hunting-dog breed whose origin is traced back to old pointing gun-dogs used to assist hunters in finding and retrieving game. This breed is popular in France, but seldom seen elsewhere. Despite the ancient background, the literature on its genetic characterization is surprisingly scarce. A recent study looked into the demography and inbreeding using pedigree records, but there is yet no report on the use of molecular markers in this breed. The aim of this work was to genotype a population of Braque Français, type Pyrénées dogs with the high-density SNP array to study the genomic diversity of the breed.
The average observed ([Formula: see text]) and expected ([Formula: see text]) heterozygosity were 0.371 ([Formula: see text]) and 0.359 ([Formula: see text]). Effective population size ([Formula: see text]) was 27.5635 runs of homozygosity (ROH) were identified with average length of 2.16 MB. A ROH shared by [Formula: see text] of the dogs was detected at the beginning of chromosome 22. Inbreeding coefficients from marker genotypes were in the range [Formula: see text]. Inbreeding estimated from ROH ([Formula: see text]) had mean [Formula: see text]), with range [0.0526, 0.225]. These results show that the Braque Français, type Pyrénées breed is a relatively inbred population, but with still sufficient genetic variability for conservation and genetic improvement.
比利牛斯短腿猎犬是一种法国猎犬品种，其起源可追溯到过去用于协助猎人寻找和寻回猎物的指示猎犬。该品种在法国很受欢迎，但在其他地方很少见。尽管有着古老的背景，但关于其遗传特征的文献却出奇地稀少。最近一项研究利用系谱记录研究了其种群统计学和近亲繁殖情况，但尚未有关于该品种使用分子标记的报道。这项工作的目的是使用高密度单核苷酸多态性（SNP）芯片对比利牛斯短腿猎犬群体进行基因分型，以研究该品种的基因组多样性。
观察到的平均杂合度（[公式：见正文]）和预期杂合度（[公式：见正文]）分别为0.371（[公式：见正文]）和0.359（[公式：见正文]）。有效种群大小（[公式：见正文]）为27.563。共鉴定出535个纯合子片段（ROH），平均长度为2.16兆字节。在22号染色体起始处检测到[公式：见正文]的犬只共享的一个ROH。基于标记基因型的近亲繁殖系数在[公式：见正文]范围内。根据ROH估计的近亲繁殖系数（[公式：见正文]）平均为[公式：见正文]，范围为[0.0526, 0.225]。这些结果表明，比利牛斯短腿猎犬品种是一个相对近亲繁殖的群体，但仍具有足够的遗传变异性用于保护和遗传改良。
