Department of Chemistry, National Taiwan University, Taipei 10617, Taiwan.
Department of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung 20224, Taiwan.
Anal Chim Acta. 2018 Mar 20;1003:42-48. doi: 10.1016/j.aca.2017.11.076. Epub 2017 Dec 12.
A graphene oxide (GO) nanosheet-modified N-nylon membrane (GOM) has been prepared and used as an extraction and spray-ionization substrate for robust mass spectrometric detection of malachite green (MG), a highly toxic disinfectant in liquid samples and fish meat. The GOM is prepared by self-deposition of GO thin film onto an N-nylon membrane, which has been used for efficient extraction of MG in aquaculture water samples or homogenized fish meat samples. Having a dissociation constant of 2.17 × 10 M, the GOM allows extraction of approximately 98% of 100 nM MG. Coupling of the GOM-spray with an ion-trap mass spectrometer allows quantitation of MG in aquaculture freshwater and seawater samples down to nanomolar levels. Furthermore, the system possesses high selectivity and sensitivity for the quantitation of MG and its metabolite (leucomalachite green) in fish meat samples. With easy extraction and efficient spray ionization properties of GOM, this membrane spray-mass spectrometry technique is relatively simple and fast in comparison to the traditional LC-MS/MS methods for the quantitation of MG and its metabolite in aquaculture products.
已制备了氧化石墨烯（GO）纳米片修饰的尼龙膜（GOM），并将其用作萃取和喷雾电离基底，用于在液体样品和鱼肉中对孔雀石绿（MG）进行稳健的质谱检测，MG 是一种具有高毒性的消毒剂。GOM 通过 GO 薄膜自沉积到尼龙膜上而制备，尼龙膜已用于从水产养殖水样或匀浆鱼肉样品中高效萃取 MG。GOM 的离解常数为 2.17×10 M，允许萃取约 100 nM MG 的 98%。将 GOM-喷雾与离子阱质谱仪耦合，可将 MG 在水产养殖淡水和海水中的定量下限降低至纳摩尔水平。此外，该系统对鱼肉样品中 MG 及其代谢物（无色孔雀石绿）的定量具有高选择性和灵敏度。与传统的 LC-MS/MS 方法相比，GOM 的膜喷雾质谱技术在水产养殖产品中定量 MG 及其代谢物时相对简单和快速，因为其具有易于萃取和高效喷雾电离的特性。
