[基于纸喷雾电离质谱法的水产养殖水中孔雀石绿定量及快速筛查]
[Quantification and fast-screening of malachite green in aquaculture water using paper spray ionization mass spectrometry].
作者信息
Zeng Dong, Yu Muqian, Wen Ruizhi, Feng Jiali
机构信息
Physical & Chemical Analysis Department, Hunan Provincial Center for Disease Control and Prevention, Changsha 410005, China.
Changsha Food and Drug Administration, Changsha 410016, China.
出版信息
Wei Sheng Yan Jiu. 2020 Jan;49(1):107-111. doi: 10.19813/j.cnki.weishengyanjiu.2020.01.019.
OBJECTIVE
A rapid quantitative technique had been developed for the screening of malachite green(MG) in aquaculture water using paper spray ionization mass spectrometry(PSI-MS).
METHODS
To rapid extract MG, chloroform and deep eutectic solvents(DESs) were used as extraction agent and co-solvent respectively. malachite green D_5(MG-D_5) was used as an internal standard to quantitative determination MG, C_(18) chromatographic column(4. 6 mm×250 mm, 5 μm).
RESULTS
The method showed a good linearity(R~2=0. 999) in the concentration range of 0. 1-10 μg/mL with the detection limit of 0. 03 μg/mL and the quantitative limit of 0. 1 μg/mL. The intra-and inter-day precisions were 3. 5% and 6. 2%, and the recovery ranged from 98. 9% to 106. 9%(n=7). The detection result by PSI-MS were in agreement with those by liquid chromatography-tandem mass spectrometry(LC-MS/MS), but the run time was only 1 min each sample.
CONCLUSION
The method is accurate and reliable. And compared with the conventional LC-MS/MS technology, it is simple, rapid and low-cost.
目的
开发一种使用纸喷雾电离质谱法（PSI-MS）快速定量检测水产养殖水中孔雀石绿（MG）的技术。
方法
分别以氯仿和深共熔溶剂（DESs）作为萃取剂和共溶剂快速萃取MG。以孔雀石绿D_5（MG-D_5）作为内标物定量测定MG，采用C_(18)色谱柱（4.6 mm×250 mm，5μm）。
结果
该方法在0.1 - 10μg/mL浓度范围内线性良好（R~2 = 0.999），检测限为0.03μg/mL，定量限为0.1μg/mL。日内和日间精密度分别为3.5%和6.2%，回收率为98.9% - 106.9%（n = 7）。PSI-MS的检测结果与液相色谱 - 串联质谱法（LC-MS/MS）一致，但每个样品的运行时间仅为1分钟。
结论
该方法准确可靠。与传统的LC-MS/MS技术相比，它简单、快速且成本低。
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