Florin Lisa, Maelegheer Karel, Muyldermans Astrid, Van Esbroeck Marjan, Nulens Eric, Emmerechts Jan
Department of Laboratory Hematology, AZ Sint-Jan Brugge-Oostende, Bruges, Belgium.
Department of Clinical Sciences, Institute of Tropical Medicine, Antwerp, Belgium.
Diagn Microbiol Infect Dis. 2018 Apr;90(4):253-256. doi: 10.1016/j.diagmicrobio.2017.12.002. Epub 2017 Dec 8.
CellaVision DM96 is a digital cell morphology system for automated classification of white and red blood cells. CellaVision Advanced RBC application (ARBCA) pre-classifies RBC in 21 categories, including parasitized RBC, and allows re-classification by the operator. In this study, the performance of the software for detection of malaria and calculation of parasitemia was evaluated and compared to microscopy (n=40). For CellaVision, both pre- and post-reclassification results were evaluated. Sensitivity was moderate, even post-reclassification (72%), due to low numbers of analyzed RBC and limited resolution of photographs. CellaVision results correlated with microscopy according to Passing-Bablok analysis, with slightly lower values for CellaVision. Within-run, between-run and inter-observer variability were acceptable. The low sensitivity of CellaVision ARBCA precludes its use as a screening technique for malaria. However, due to its good correlation with microscopy and short turn-around-times, it may be useful in follow-up of parasitemia. Larger studies are required to confirm these findings.
赛乐维斯DM96是一种用于自动分类白细胞和红细胞的数字细胞形态学系统。赛乐维斯高级红细胞应用程序（ARBCA）可将红细胞预分类为21种类型，包括寄生红细胞，并允许操作人员进行重新分类。在本研究中，评估了该软件检测疟疾和计算疟原虫血症的性能，并与显微镜检查（n = 40）进行了比较。对于赛乐维斯系统，评估了重新分类前后的结果。由于分析的红细胞数量较少且照片分辨率有限，即使在重新分类后，灵敏度也处于中等水平（72%）。根据帕辛-巴布洛赫分析，赛乐维斯系统的结果与显微镜检查结果相关，但赛乐维斯系统的值略低。批内、批间和观察者间的变异性均可接受。赛乐维斯ARBCA的低灵敏度使其不能用作疟疾筛查技术。然而，由于其与显微镜检查具有良好的相关性且周转时间短，它可能有助于疟原虫血症的随访。需要更大规模的研究来证实这些发现。
