Hervent A-S, Godefroid M, Cauwelier B, Billiet J, Emmerechts J
Department of Laboratory Hematology, AZ Sint-Jan, Bruges, Belgium.
Int J Lab Hematol. 2015 Oct;37(5):588-96. doi: 10.1111/ijlh.12363. Epub 2015 Apr 28.
The CellaVision Advanced Red Blood Cell (RBC) Software Application is a new software for advanced morphological analysis of RBC, which automatically performs a preliminary characterization and grouping of RBC into 21 morphological categories, including schistocytes. Upon automated classification, the software offers the possibility of reclassification of RBC by the operator. The aim of this study was to evaluate the schistocyte analysis by the CellaVision Advanced RBC Application.
Schistocyte counts were evaluated comparing the automated count on a CellaVision DM96, both before and after reclassification, with the reference manual microscopic method according to the ICSH criteria. Thirty-six samples of hospitalized patients and 40 samples of controls were analyzed.
Within-run, between-run and between-observer coefficients of variation were lower when counted with the CellaVision compared to the manual microscopic count. The very high sensitivity but rather poor specificity implicates the need for reclassification by the operator, following automated analysis. After reclassification, method comparison studies revealed good agreement with the manual microscopic method, with however slightly higher values of schistocytes for the automated analysis.
The CellaVision Advanced RBC Software Application provides a sensitive and reproducible measurement of schistocytes in peripheral blood, but still requires manual revision. Furthermore, it is an easy-to-use software and an excellent teaching tool that might contribute to standardization in the investigation of schistocyte-related conditions.
CellaVision先进红细胞（RBC）软件应用程序是一款用于红细胞高级形态分析的新软件，它能自动对红细胞进行初步特征描述，并将其分为21种形态类别，包括裂红细胞。在自动分类后，该软件为操作人员提供了对红细胞重新分类的可能性。本研究的目的是评估CellaVision先进红细胞应用程序对裂红细胞的分析。
根据国际血液学标准化委员会（ICSH）标准，通过将CellaVision DM96上自动计数（重新分类前后）与参考手工显微镜检查方法进行比较，评估裂红细胞计数。分析了36例住院患者样本和40例对照样本。
与手工显微镜计数相比，使用CellaVision计数时，批内、批间和观察者间变异系数更低。极高的灵敏度但相对较差的特异性意味着在自动分析后需要操作人员进行重新分类。重新分类后，方法比较研究显示与手工显微镜检查方法具有良好的一致性，不过自动分析的裂红细胞值略高。
CellaVision先进红细胞软件应用程序对外周血中的裂红细胞提供了一种灵敏且可重复的测量方法，但仍需要人工修正。此外，它是一款易于使用的软件和出色的教学工具，可能有助于裂红细胞相关病症研究的标准化。
