Bosca L, Rousseau G G, Hue L
Proc Natl Acad Sci U S A. 1985 Oct;82(19):6440-4. doi: 10.1073/pnas.82.19.6440.
Incubation of chicken embryo fibroblasts with mitogenic concentrations of insulin for 24 hr or with the tumor promoter phorbol 12-myristate 13-acetate for 6 hr stimulated lactate release and 3-O-methylglucose uptake. Insulin also increased the Vmax of 6-phosphofructo-1-kinase (ATP:D-fructose-6-phosphate 1-phosphotransferase, EC 2.7.1.11). Both agents increased the concentration of fructose 2,6-bisphosphate and the activity of 6-phosphofructo-2-kinase (EC 2.7.1.-), the enzyme that catalyzes the synthesis of this stimulator of 6-phosphofructo-1-kinase. These changes provide an explanation for the stimulation of glycolysis by insulin and phorbol esters. In contrast to the situation in rat liver, fructose 2,6-bisphosphate concentration did not decrease after cyclic AMP treatment. Incubation of cells with phorbol ester analogues or with glycerol derivatives that are known to stimulate, or to bind to, protein kinase C did increase the concentration of fructose 2,6-bisphosphate, suggesting that the stimulation of 6-phosphofructo-2-kinase by phorbol 12-myristate 13-acetate is mediated by protein kinase C.
用促有丝分裂浓度的胰岛素孵育鸡胚成纤维细胞24小时，或用肿瘤启动子佛波醇12 - 肉豆蔻酸酯13 - 乙酸酯孵育6小时，可刺激乳酸释放和3 - O - 甲基葡萄糖摄取。胰岛素还增加了6 - 磷酸果糖 - 1 - 激酶（ATP：D - 果糖 - 6 - 磷酸1 - 磷酸转移酶，EC 2.7.1.11）的Vmax。这两种物质都增加了果糖2,6 - 二磷酸的浓度以及6 - 磷酸果糖 - 2 - 激酶（EC 2.7.1.-）的活性，该酶催化合成这种6 - 磷酸果糖 - 1 - 激酶的刺激物。这些变化为胰岛素和佛波酯对糖酵解的刺激作用提供了解释。与大鼠肝脏中的情况相反，环磷酸腺苷处理后果糖2,6 - 二磷酸浓度并未降低。用已知能刺激或结合蛋白激酶C的佛波酯类似物或甘油衍生物孵育细胞，确实会增加果糖2,6 - 二磷酸的浓度，这表明佛波醇12 - 肉豆蔻酸酯13 - 乙酸酯对6 - 磷酸果糖 - 2 - 激酶的刺激作用是由蛋白激酶C介导的。
