Department of Ophthalmology, Koshigaya Hospital, Dokkyo Medical University, Koshigaya, Saitama, Japan.
Department of Ophthalmology, Koshigaya Hospital, Dokkyo Medical University, Koshigaya, Saitama, Japan; Lively Eye Clinic, Soka, Saitama, Japan.
Exp Eye Res. 2018 Mar;168:28-32. doi: 10.1016/j.exer.2018.01.001. Epub 2018 Jan 6.
Antioxidants can decrease oxidative damage and prevent age-related ocular disease. Our previous investigation on human aqueous humor following intake of a lutein-containing antioxidant supplement reported an increase in the scavenging activity of superoxide in both genders and an increase in the amount of hydrogen peroxide (HO) in females. Aquaporin 8 (AQP8) is a diffusion facilitator of HO and glutathione peroxidase (Gpx) is a HO scavenging enzyme. The correlation between AQP8 and Gpx may be the key to determining how oxidative stress in the aqueous humor affects the lens after intake of antioxidant supplements. In this study, 24 patients with the same grade of binocular cataract were included. Anterior capsule samples, including lens epithelial cells (LECs), were collected during cataract surgery before (as pre-intake samples) and after 6 weeks of oral intake of Ocuvite Lutein (as post-intake samples). The mRNA expression of APQ8 and Gpx was measured using real-time polymerase chain reaction. Among males, AQP8 expression decreased significantly after the supplementation (P = .03), while there was no statistical change among females. AQP8 expression was significantly correlated to that of Gpx in post-intake samples among females (R = 0.69, P = .02), while no correlation was evident among males. The results suggest antioxidant supplementation may work by different mechanisms on LECs between genders. After supplementation, a decrease in AQP8 in LECs may inhibit the influx of HO from the aqueous humor in males. In females however, the correlation between AQP8 and Gpx in LECs may indicate an increase in Gpx activity following the influx of HO from the aqueous humor and further scavenging of HO.
抗氧化剂可以减少氧化损伤，预防与年龄相关的眼部疾病。我们之前的研究报告称，在男性和女性中，摄入含有叶黄素的抗氧化补充剂后，人眼房水中的超氧化物清除活性增加，女性眼水中的过氧化氢（HO）量增加。水通道蛋白 8（AQP8）是 HO 和谷胱甘肽过氧化物酶（Gpx）的扩散促进剂。AQP8 和 Gpx 之间的相关性可能是确定抗氧化补充剂摄入后眼水中的氧化应激如何影响晶状体的关键。在这项研究中，纳入了 24 名双眼白内障程度相同的患者。在白内障手术期间采集前囊样本，包括晶状体上皮细胞（LEC），并在术前（作为摄入前样本）和口服 Ocuvite Lutein 6 周后（作为摄入后样本）进行收集。使用实时聚合酶链反应测量 AQP8 和 Gpx 的 mRNA 表达。在男性中，补充后 AQP8 表达显著降低（P=0.03），而女性中则没有统计学变化。女性摄入后样本中 AQP8 表达与 Gpx 显著相关（R=0.69，P=0.02），而男性则无明显相关性。结果表明，抗氧化补充剂对男女 LEC 可能具有不同的作用机制。补充后，LEC 中 AQP8 的减少可能会抑制来自房水的 HO 的流入，而在女性中，LEC 中 AQP8 和 Gpx 之间的相关性可能表明来自房水的 HO 流入后 Gpx 活性增加，进一步清除 HO。
