Department of Ophthalmology, Kanazawa Medical University, Ishikawa 9200293, Japan.
Supplement Development & Promotion Group, Santen Pharmaceutical Co., Ltd., Tokyo 1030022, Japan.
Biomed Res Int. 2020 May 19;2020:9204620. doi: 10.1155/2020/9204620. eCollection 2020.
Age-related cataract (ARC) is the major cause of blindness worldwide. The most significant factors are the maximal exposure of the eye lens to environmental stressors, including oxidative and glycative load. The administration of antioxidant and antiglycative supplements may reduce the risk of cataract progression. In this study, the effects of lutein (LU) and water chestnut ( Roxb.) extract (TBE) on cataracts and the expression of antioxidant-related genes were assessed in Shumiya cataract rats (SCRs). LU+TBE or castor oil (COil) as a control was administered to 6- or 9-week-old cataractous SCRs and noncataractous SCRs via a feeding needle for 3 or 4 weeks. Five-week-old SCRs were provided access to solid regular chow containing LU, TBE, LU+TBE, or the same chow without LU and/or TBE for 3 weeks. Lenses from all rats were then extracted and photographed. The right eyes of the rats were processed for histological observation, and the left eyes were used for total RNA extraction from lens epithelial cells (LEC). The mRNA levels of antioxidant proteins, peroxiredoxin 6, and catalase were examined using real-time quantitative polymerase chain reaction. Lens opacity appeared in all cataractous SCRs that began receiving LU+TBE at 9 weeks of age. However, compared to the COil group, lens opacity was decreased in the cataractous LU+TBE SCRs in all experiments. The mRNA expression levels of peroxiredoxin 6 and catalase in LECs of cataractous SCRs and cultured human LECs increased after the administration of LU+TBE. Collectively, our results highlight the anticataract and antioxidative effects of LT+TBE in SCRs. LT+TBE supplementation may, thus, be useful in delaying cataract progression.
年龄相关性白内障（ARC）是全球范围内失明的主要原因。最重要的因素是眼睛晶状体受到环境应激物（包括氧化应激和糖基化应激）的最大暴露。抗氧化和抗糖基化补充剂的管理可能会降低白内障进展的风险。在这项研究中，评估了叶黄素（LU）和马蹄（Roxb.）提取物（TBE）对 Shumiya 白内障大鼠（SCR）白内障和抗氧化相关基因表达的影响。LU+TBE 或蓖麻油（COil）作为对照，通过喂食针给予 6 或 9 周龄白内障 SCR 和非白内障 SCR 3 或 4 周。5 周龄 SCR 可获得含有 LU、TBE、LU+TBE 或不含 LU 和/或 TBE 的相同饲料 3 周。然后从所有大鼠中提取晶状体并拍照。对大鼠右眼进行组织学观察，左眼用于从晶状体上皮细胞（LEC）中提取总 RNA。使用实时定量聚合酶链反应检查抗氧化蛋白、过氧化物酶 6 和过氧化氢酶的 mRNA 水平。所有开始在 9 周龄时接受 LU+TBE 的白内障 SCR 均出现晶状体混浊。然而，与 COil 组相比，所有实验中白内障 LU+TBE SCR 的晶状体混浊程度均降低。白内障 SCR 和培养的人 LEC 中 LE 的过氧化物酶 6 和过氧化氢酶的 mRNA 表达水平在给予 LU+TBE 后增加。总之，我们的结果强调了 LT+TBE 在 SCR 中的抗白内障和抗氧化作用。LT+TBE 补充剂可能有助于延缓白内障进展。
