Feng Yalong, Feng Juantao, Zheng Hanxue, Wang Wenjun, Chen Fulin, Yu Yuan, Cui Jihong
Lab of Tissue Engineering, College of Life Sciences, Northwest University, Xi'an 710069, PR China.
Lab of Tissue Engineering, College of Life Sciences, Northwest University, Xi'an 710069, PR China; Provincial Key Laboratory of Biotechnology of Shaanxi, Xi'an 710069, PR China; Key Laboratory of Resource Biology and Biotechnology in Western China Ministry of Education, Xi'an 710069, PR China.
Gene. 2018 Mar 20;647:226-234. doi: 10.1016/j.gene.2018.01.012. Epub 2018 Jan 6.
The cysteine- and glycine-rich protein (CRP) family members, including the cysteine- and glycine-rich protein 1 (CSRP1), cysteine- and glycine-rich protein 2 (CSRP2), and the cysteine- and glycine-rich protein 3 (CSRP3), have exhibited various cellular functions during cell development and differentiation. However, the sequences of the three CSRP genes and their functions are still poorly understood in newts. In this study, we cloned the complete open reading frame (ORF) sequences of the three CSRP genes from the Chinese fire-bellied newt, Cynops orientalis (C. orientalis). The complete ORF sequences of Co-CSRP1, Co-CSRP2, and Co-CSRP3 were 582, 582, and 576bp, respectively, and encoded 193, 193, and 191 amino acids, respectively. The deduced amino acid sequences of the three CRP members showed high similarities with that of other species, particularly, with amphibians. Co-CSRP1 was highly expressed in the kidney, limb, and stomach, however, the expression was low in the spleen, heart, intestine, liver, and tail (P<0.05). The mRNA expression of Co-CSRP2 was higher in the kidney and heart than that in other organs (P<0.05). It was observed that Co-CSRP3 was only expressed in the heart, limb, and tail. The mRNA expression of Co-CSRP1 and Co-CSRP3 was lower in the digits in comparison to other limb segments. However, there was no significant difference of Co-CSRP2 mRNA expression in the four limb segments. The Co-CSRP1 and Co-CSRP2 mRNA expressions were significantly increased, whereas the expression of Co-CSRP3 was remarkably decreased during the limb regeneration. This study will provide useful information for further elucidating the role of Co-CSRP genes during newt limb regeneration.
富含半胱氨酸和甘氨酸的蛋白（CRP）家族成员，包括富含半胱氨酸和甘氨酸的蛋白1（CSRP1）、富含半胱氨酸和甘氨酸的蛋白2（CSRP2）以及富含半胱氨酸和甘氨酸的蛋白3（CSRP3），在细胞发育和分化过程中展现出多种细胞功能。然而，蝾螈中这三个CSRP基因的序列及其功能仍知之甚少。在本研究中，我们从中国林蛙（东方蝾螈）中克隆了这三个CSRP基因的完整开放阅读框（ORF）序列。Co-CSRP1、Co-CSRP2和Co-CSRP3的完整ORF序列分别为582、582和576bp，分别编码193、193和191个氨基酸。这三个CRP成员的推导氨基酸序列与其他物种，特别是两栖动物的序列具有高度相似性。Co-CSRP1在肾脏、肢体和胃中高表达，然而，在脾脏、心脏、肠道、肝脏和尾巴中的表达较低（P<0.05）。Co-CSRP2的mRNA表达在肾脏和心脏中高于其他器官（P<0.05）。观察到Co-CSRP3仅在心脏、肢体和尾巴中表达。与其他肢体节段相比，Co-CSRP1和Co-CSRP3的mRNA表达在指节中较低。然而，Co-CSRP2的mRNA表达在四个肢体节段中没有显著差异。在肢体再生过程中，Co-CSRP1和Co-CSRP2的mRNA表达显著增加，而Co-CSRP3的表达显著降低。本研究将为进一步阐明Co-CSRP基因在蝾螈肢体再生中的作用提供有用信息。
