Xu Xuewen, Qiu Haifang, Du Zhi-Qiang, Fan Bin, Rothschild Max F, Yuan Fan, Liu Bang
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, China.
Mol Biol Rep. 2010 Jan;37(1):451-9. doi: 10.1007/s11033-009-9632-1. Epub 2009 Jul 26.
CRP3 is the muscle-specific form of the cysteine and glycine-rich protein family and plays an important role in myofiber differentiation. Here we isolated and characterized its coding gene CSRP3 from porcine muscle. Phylogenic analyses demonstrated that CSRP3 diverged first and is distinguished from two other members, CSRP1 and CSRP2. CSRP3 mRNA was up-regulated during the development of porcine embryonic skeletal muscle, indicating its potential importance in muscle growth. Genetic variant analyses detected multiple variations in an approximately 400 bp region covering exon 4 and its downstream intron, and two haplotypes were identified by sequencing. One of synonymous substitutions C1924T was used for linkage and association analyses. It was revealed that the substitution of C1924T had significant associations with firmness (P < 0.01), Lab Loin pH, Off Flavor Score and Water Holding Capacity (P < 0.05), and a suggestive effect (P < 0.1) on Flavor Score and Average Glycolytic Potential in a Berkshire x Yorkshire F2 population. The association analyses results agreed with the gene's localization to a QTL region for meat quality traits on porcine chromosome 2p14-17 demonstrated by both linkage mapping and RH mapping. These results provide fundamental evidence for CSRP3 as a functional candidate gene affecting pig meat quality.
CRP3是富含半胱氨酸和甘氨酸的蛋白质家族的肌肉特异性形式，在肌纤维分化中起重要作用。在此，我们从猪肌肉中分离并鉴定了其编码基因CSRP3。系统发育分析表明，CSRP3首先分化，与另外两个成员CSRP1和CSRP2不同。CSRP3 mRNA在猪胚胎骨骼肌发育过程中上调，表明其在肌肉生长中的潜在重要性。遗传变异分析在覆盖外显子4及其下游内含子的约400 bp区域检测到多个变异，并通过测序鉴定出两种单倍型。同义替换C1924T之一用于连锁和关联分析。结果表明，在伯克希尔×约克夏F2群体中，C1924T的替换与嫩度（P < 0.01）、腰大肌pH值、异味评分和持水力（P < 0.05）显著相关，对风味评分和平均糖酵解潜能有提示性影响（P < 0.1）。关联分析结果与通过连锁图谱和辐射杂种图谱证明的该基因定位于猪2号染色体p14 - 17上肉质性状的QTL区域一致。这些结果为CSRP3作为影响猪肉品质的功能候选基因提供了基础证据。
