Lehtonen L, Vainio O, Eerola E, Toivanen P
Transplantation. 1985 Oct;40(4):398-404. doi: 10.1097/00007890-198510000-00011.
Transplantation of allogeneic bursal cells into cyclophosphamide-treated, immunodeficient chickens is a useful experimental model for analyzing the mechanisms of transplantation tolerance, especially because transplanted bursal cells do not produce graft-versus-host disease. In this study we have determined B-lymphoid chimerism in various lymphoid organs after transplantation of allogeneic bursal stem cells or postbursal cells, and used a variety of tests to determine presence of immunological tolerance. Transplanted bursal stem cells induced a state of stable chimerism that could easily be detected in peripheral blood and other lymphoid organs. Chimerism induced by postbursal cells was low in peripheral blood, but clearly observable in other lymphoid organs, especially in spleen and thymus. Both bursal and postbursal cells induced specific unresponsiveness to donor-line alloantigens. Bursal cell recipients accepted donor line skin grafts--and their graft-versus-host reactivity, as assayed by embryonal splenomegaly, and mixed lymphocyte reactivity against donor line alloantigens were significantly decreased. Despite differences in chimerism, a strong transplantation tolerance was readily induced with bursal stem cells and with postbursal cells.
将同种异体法氏囊细胞移植到经环磷酰胺处理的免疫缺陷鸡中，是分析移植耐受机制的一种有用的实验模型，特别是因为移植的法氏囊细胞不会产生移植物抗宿主病。在本研究中，我们测定了同种异体法氏囊干细胞或法氏囊后细胞移植后各种淋巴器官中的B淋巴细胞嵌合现象，并使用了多种测试来确定免疫耐受的存在。移植的法氏囊干细胞诱导了一种稳定的嵌合状态，在外周血和其他淋巴器官中很容易检测到。法氏囊后细胞诱导的嵌合在外周血中较低，但在其他淋巴器官中明显可见，尤其是在脾脏和胸腺中。法氏囊细胞和法氏囊后细胞均诱导对供体系同种异体抗原的特异性无反应性。法氏囊细胞受体接受供体系皮肤移植，并且通过胚胎脾肿大测定的它们的移植物抗宿主反应性以及针对供体系同种异体抗原的混合淋巴细胞反应性均显著降低。尽管嵌合现象存在差异，但法氏囊干细胞和法氏囊后细胞都很容易诱导出强烈的移植耐受。
