Toivanen P, Toivanen A, Sorvari T
Proc Natl Acad Sci U S A. 1974 Mar;71(3):957-61. doi: 10.1073/pnas.71.3.957.
Transplantation of allogeneic cells from bursa of Fabricius into cyclophosphamide-treated, immunodeficient chicks resulted in immunological tolerance to donor line skin grafts; graft-versus-host disease did not occur. Allogeneic bursal stem cells taken from 3-day-old donors induced restoration of bursal morphology, of antibody formation to Brucella abortus and of occurrence of pyroninophilic cells and immunoglobulin-bearing cells in the peripheral lymphoid tissues. Secondary response to sheep red blood cells and production of germinal centers were not restored. Transplantation of histocompatible bursal stem cells resulted in a complete reconstitution of the bursa-dependent lymphoid system, both in function and in morphology. Allogeneic postbursal stem cells taken from the bursa of 10-week-old donors had a reconstitutive effect only on the production of antibodies to Brucella. Transplanted stem cells maintained their functional potential in the allogeneic environment, since, when transferred back to histocompatible hosts, they displayed normal function. These findings indicate that a complete functional and morphological restoration of the bursa-dependent immune system cannot be achieved without identity of the donor and recipient at loci near to or identical with those determining the major histocompatibility antigens, even though graft-versus-host disease is avoided. This identity permits a full cooperation of the donor and host cells.
将法氏囊的同种异体细胞移植到经环磷酰胺处理的免疫缺陷雏鸡体内，可诱导其对供体品系皮肤移植物产生免疫耐受；未发生移植物抗宿主病。取自3日龄供体的同种异体法氏囊干细胞可诱导法氏囊形态恢复、对流产布鲁氏菌抗体形成恢复以及外周淋巴组织中嗜派洛宁细胞和含免疫球蛋白细胞出现。对绵羊红细胞的二次应答和生发中心的产生未恢复。组织相容性法氏囊干细胞的移植导致法氏囊依赖性淋巴系统在功能和形态上完全重建。取自10周龄供体法氏囊的同种异体囊后干细胞仅对布鲁氏菌抗体产生有重建作用。移植的干细胞在同种异体环境中保持其功能潜能，因为当重新移植到组织相容性宿主时，它们表现出正常功能。这些发现表明，即使避免了移植物抗宿主病，但如果供体和受体在决定主要组织相容性抗原的位点附近或相同的位点上不具有同一性，就无法实现法氏囊依赖性免疫系统在功能和形态上的完全恢复。这种同一性允许供体细胞和宿主细胞充分合作。
