Haralambie E, Littmann K, Edenharder R, Linzenmeier G
Zentralbl Bakteriol Mikrobiol Hyg A. 1985 May;259(3):359-66.
The relation between cholecystectomy and colon carcinogenesis has not been fully elucidated. As bacteria may be involved in the carcinogenic process, we investigated the effect of cholecystectomy and dimethylhydrazine (DMH) administration to SPF NMRI mice with regard to tumour genesis and bacterial colonisation of the intestine. It results from this study that cholecystectomy does not influence tumour genesis and that 6-7 months post operationem and DMH administration tumours and bacteria originally not found in the animals develop: clostridia, eubacteria spec. which cannot be differentiated and E. lentum. Theses changes appear in group II of mice (laparotomy and DMH) and group III (cholecystectomy and DMH), but not in group I (controls). From the results of this study we cannot conclude whether the tumours or the new bacteria appeared first. Biochemical investigations of C. innocuum, C. paraputrificum and C. tertium indicated that these bacteria metabolised bile acids by a specific metabolic step only but not produced carcinogenic substances themselves. If bacteria are involved in tumorgenesis, different species may be involved producing a carcinogenic environment by metabolic chain reactions. We know of such a bacterial collaboration in anaerobic infections.
胆囊切除术与结肠癌发生之间的关系尚未完全阐明。由于细菌可能参与致癌过程，我们研究了胆囊切除术和给无特定病原体（SPF）的NMRI小鼠注射二甲基肼（DMH）对肿瘤发生和肠道细菌定植的影响。这项研究结果表明，胆囊切除术不影响肿瘤发生，并且在术后6 - 7个月以及注射DMH后，动物体内原本不存在的肿瘤和细菌开始出现：梭菌属、真细菌属（无法区分的种类）以及迟缓埃格特菌。这些变化出现在II组小鼠（剖腹术和DMH）和III组（胆囊切除术和DMH）中，但未出现在I组（对照组）中。从这项研究结果我们无法得出肿瘤或新细菌谁先出现的结论。对无害梭菌、副腐败梭菌和第三梭菌的生化研究表明，这些细菌仅通过特定代谢步骤代谢胆汁酸，自身并不产生致癌物质。如果细菌参与肿瘤发生，不同种类的细菌可能通过代谢连锁反应参与形成致癌环境。我们知道在厌氧感染中存在这样的细菌协同作用。
