Department of General and Environmental Microbiology, Poznań University of Life Sciences, Poznań, Poland.
Department of Biochemistry and Biotechnology, Poznań University of Life Sciences, Poznań, Poland.
Pol J Microbiol. 2017 Sep 27;66(3):375-381. doi: 10.5604/01.3001.0010.4875.
Investigations of bacterial communities and characterization of mineralogy of the environment in the Złoty Stok As-Au deposit were carried out. PXRD analysis revealed the presence of picropharmacolite as the most common secondary arsenic mineral in the mine. Total DNA was extracted from slime streams or slime biofilms samples to investigate the bacterial communities. PCR amplification of 16S rDNA was performed followed by subcloning of its products. Over 170 clones were analyzed by means of RFLP method. Eight group of clones representing different restriction patterns were identified. The nucleotide sequences of their inserts suggest that bacteria present in the mine environment belong to: Flavobacteria, Sphingobacteriia, Bacteroides, Proteobacteria, Mollicutes and Firmicutes. The metagenomic approach allows to demonstrate a higher diversity of microbiota than classical microbiological studies of cultivable isolates.
对 Złoty Stok 金银矿床中的细菌群落进行了调查，并对环境的矿物学特性进行了描述。X 射线粉末衍射（PXRD）分析表明，在该矿中，最常见的次生砷矿物是针铁矿绿泥石。从矿浆或矿浆生物膜样品中提取总 DNA，以研究细菌群落。对 16S rDNA 进行 PCR 扩增，然后对其产物进行亚克隆。通过 RFLP 方法分析了 170 多个克隆。根据不同的限制模式，鉴定出 8 组克隆。它们插入片段的核苷酸序列表明，矿坑环境中的细菌属于：黄杆菌、鞘脂单胞菌、拟杆菌、变形菌、柔膜菌和厚壁菌。与可培养分离物的经典微生物学研究相比，宏基因组学方法可以证明微生物组的多样性更高。
