Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
Sci Total Environ. 2013 Sep 1;461-462:330-40. doi: 10.1016/j.scitotenv.2013.04.087. Epub 2013 Jun 4.
In this paper we characterize the biofilm community from an ancient Złoty Stok gold and arsenic mine. Bacterial diversity was examined using a culture-independent technique based on 16S rRNA gene amplification, cloning and sequencing. We show that unexpectedly the microbial diversity of this community was extremely high (more than 190 OTUs detected), with the most numerous members from Rhizobiales (α-Proteobacteria). Although the level of rock biofilm diversity was similar to the microbial mat community we have previously characterized in the same adit, its taxonomic composition was completely different. Detailed analysis of functional arrA and aioA genes, chemical properties of siderophores found in pore water as well as the biofilm chemical composition suggest that the biofilm community contributes to arsenic pollution of surrounding water in a biogeochemical cycle similar to the one observed in bacterial mats. To interpret our results concerning the biological arsenic cycle, we applied the theory of ecological pyramids of Charles Elton.
本文对来自古老的 Złoty Stok 金矿和砷矿的生物膜群落进行了描述。使用基于 16S rRNA 基因扩增、克隆和测序的非培养技术研究了细菌多样性。我们发现，这个群落的微生物多样性出人意料地非常高（检测到的 OTU 超过 190 个），其中数量最多的成员来自根瘤菌目（α-变形菌）。尽管岩石生物膜的多样性水平与我们之前在同一个入口处描述的微生物席群落相似，但它们的分类组成完全不同。详细分析功能 arrA 和 aioA 基因、孔水中发现的铁载体的化学性质以及生物膜的化学成分表明，生物膜群落通过类似于在细菌席中观察到的生物地球化学循环，促进了周围水体的砷污染。为了解释我们关于生物砷循环的结果，我们应用了查尔斯·埃尔顿的生态金字塔理论。
