贝尔格莱德地区蓖麻硬蜱中伯氏疏螺旋体复合群的流行情况及昆虫学风险指数评估

Prevalence of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks and assessment of entomological risk index at localities in Belgrade.

作者信息

Krstić Milena, Stajković Novica, Lazić Srđan

出版信息

Vojnosanit Pregl. 2016 Sep;73(9):817-24. doi: 10.2298/VSP150115069K.

PMID:29320145

Abstract

BACKGROUND/AIM: The first case of human Lyme borreliosis (LB) in Serbia was recorded in 1987. The number of reported LB cases has increased in the past decade. The aim of this study was to estimate the density of Ixodes ricinus (I. ricinus) ticks, the prevalence of Borrelia burgdorferi sensu lato (B. burgdorferi) in them, and entomological risk index (ERI) at 19 Belgrade localities which were grouped into three categories (forests, parkforests, parks). The values of ERI were compared with the number of tick bites in humans.

METHODS

Ticks were collected monthly by using the flag hours method and the infection rate was determined by using dark field microscopy. The ERI value was calculated for each locality where the ticks were collected. The related data about tick bites was obtained from the patient protocol of the Institute of Epidemiology, Military Medical Academy, Belgrade.

RESULTS

The total number of collected ticks, the number of nymphs and the infection rates of the nymphs were significantly higher in forests (p < 0.05) than park-forests and parks. Statistically, the ERI value was significantly higher in forests than parks of Belgrade (χ2 = 7.78, p < 0.01). In March and July, the ERI value was also significantly higher in forests, than park-forests (p < 0.01) and parks (p < 0.01). May was the month with the highest ERI value in each ecological category (forests p < 0.05; park-forests p < 0.01; parks p < 0.001). However, the number of tick bites in humans did not correlate with ERI values.

CONCLUSION

The obtained results indicate that the risk of tick bite and human exposure to B. burgdorferi sensu lato is present at all selected localities in Belgrade. For a more comprehensive Lyme disease risk assessment the method of entomological risk index assessment should be combined with other methods, taking into consideration all tick stages and the behaviour and habits of people who may get infected B. burgdorferi sensu lato.

摘要

背景/目的：1987年塞尔维亚记录了首例人类莱姆病（LB）病例。在过去十年中，报告的LB病例数量有所增加。本研究的目的是估计19个贝尔格莱德地区蓖麻硬蜱（I. ricinus）的密度、其中伯氏疏螺旋体狭义种（B. burgdorferi）的患病率以及昆虫学风险指数（ERI），这些地区分为三类（森林、公园森林、公园）。将ERI值与人类蜱叮咬数量进行比较。

方法

每月采用旗小时法收集蜱虫，并通过暗视野显微镜确定感染率。计算每个收集蜱虫的地区的ERI值。有关蜱叮咬的相关数据来自贝尔格莱德军事医学科学院流行病学研究所的患者协议。

结果

森林中收集到的蜱虫总数、若虫数量和若虫感染率显著高于公园森林和公园（p < 0.05）。从统计学上讲，贝尔格莱德森林中的ERI值显著高于公园（χ2 = 7.78，p < 0.01）。在3月和7月，森林中的ERI值也显著高于公园森林（p < 0.01）和公园（p < 0.01）。5月是每个生态类别中ERI值最高的月份（森林p < 0.05；公园森林p < 0.01；公园p < 0.001）。然而，人类蜱叮咬的数量与ERI值无关。